Femtosecond laser photodisruption of primate trabecular meshwork: an ex vivo study

Investigative Ophthalmology & Visual Science
Hiroshi NakamuraDeepak P Edward

Abstract

The femtosecond laser has been realized as an advantageous tool for micromachining, and the feasibility of employing it for surgical procedures has been investigated. A prior study demonstrated dose-dependent femtosecond laser photodisruption of peripheral corneal tissue through a gonioscopic lens in enucleated porcine eyes and of the trabecular meshwork (TM) in human corneoscleral rim tissues, with little collateral damage. The present study was undertaken to extend these efforts to ex vivo primate eyes. Photodisruption of the TM in enucleated baboon eyes and human donor eyes was performed with a gonioscopic lens and a custom femtosecond laser ablation delivery system. Laser ablation was executed with a Titanium:Sapphire laser (800-nm wavelength), focused with a 0.15-NA lens, with the following settings: pulse duration, 45 fs; pulse energy, 60 to 480 microJ; pulse repetition rate, 1 kHz, total exposure time, 0.001 to 0.3 seconds. The laser lesions were evaluated by two-photon microscopy. Laser-induced lesions were consistently observed in the TM of the baboon and human eyes and visualized by two-photon microscopy. These oblique, trough-shaped lesions, which did not penetrate the juxtacanalicular region, had sharp edges and sho...Continue Reading

Citations

Dec 20, 2012·Investigative Ophthalmology & Visual Science·Jose M GonzalezJames C H Tan
Oct 30, 2010·Journal of Vacuum Science & Technology. A, Vacuum, Surfaces, and Films : an Official Journal of the American Vacuum Society·Slobodan MilasinovicLuke Hanley
Feb 27, 2014·Lasers in Medical Science·Mehra S SidhuIl-Hong Park
Jan 25, 2011·Biomedical Optics Express·Youbo ZhaoRobert J Gordon
Jan 30, 2014·Natural Product Reports·Chhavi Bhardwaj, Luke Hanley
Aug 19, 2021·Translational Vision Science & Technology·Eric MikulaTibor Juhasz

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