PMID: 9159127May 27, 1997Paper

Femtosecond time resolution in x-ray diffraction experiments

Proceedings of the National Academy of Sciences of the United States of America
R Neutze, J Hajdu

Abstract

This paper presents the theoretical background for a synthesis of femtosecond spectroscopy and x-ray diffraction. When a diffraction quality crystal with 0.1-0.3 mm overall dimensions is photoactivated by a femtosecond laser pulse (physical length = 0.3 microm), the evolution of molecules at separated points in the crystal will not be simultaneous because a finite time is required for the laser pulse to propagate through the body of the crystal. Utilizing this lack of global crystal synchronization, topographic x-ray diffraction may enable femtosecond temporal resolution to be achieved from reflection profiles in the diffraction pattern with x-ray exposures of picosecond or longer duration. Such x-ray pulses are currently available, and could be used to study femtosecond reaction dynamics at atomic resolution on crystals of both small- and macromolecules. A general treatment of excitation and diffraction geometries in relation to spatial and temporal resolution is presented.

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Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·J L Martin, M H Vos
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Citations

Nov 5, 1997·Current Opinion in Structural Biology·K Moffat, Z Ren
Nov 18, 1998·Current Opinion in Structural Biology·B L Stoddard
Feb 19, 2000·Current Opinion in Chemical Biology·G A Petsko, D Ringe
Aug 12, 1998·Nature Structural Biology·K Moffat
Nov 14, 1997·Nature Structural Biology·J R Helliwell
Jul 11, 2006·The Journal of Chemical Physics·Panayiotis GeorgiouRichard Neutze
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Thomas P SakmarElias S Awad
Apr 16, 2005·Science·A M LindenbergJ B Hastings

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