Fenpyroximate binds to the interface between PSST and 49 kDa subunits in mitochondrial NADH-ubiquinone oxidoreductase

Biochemistry
Yusuke ShiraishiHideto Miyoshi

Abstract

Using a photoaffinity labeling technique, Nakamaru-Ogiso et al. demonstrated that fenpyroximate, a strong inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I), binds to the ND5 subunit [Nakamaru-Ogiso, E., et al. (2003) Biochemistry 42, 746-754]. Considering that the main body of the ND5 subunit composed of transmembrane helixes 1-15 is located at the distal end of the membrane domain [Efremov, R. G., et al. (2010) Nature 465, 441-445], however, their result may be questionable. Because establishing the number and location of inhibitors and/or quinone binding sites in the membrane domain is necessary to elucidate the function of the enzyme, it is critical to clarify whether there is an additional inhibitor and/or quinone binding site besides the interface between the hydrophilic and membrane domains. We therefore performed photoaffinity labeling experiments using two newly synthesized fenpyroximate derivatives [[(125)I]-4-azidophenyl fenpyroximate ([(125)I]APF) and [(125)I]-3-azido-5-iodobenzyl fenpyroximate ([(125)I]AIF)] possessing a photoreactive azido group at and far from the pharmacophoric core moiety, respectively. Doubled sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed th...Continue Reading

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Citations

Jun 30, 2014·Journal of Bioenergetics and Biomembranes·Motoaki SatoTakao Yagi
Jul 6, 2014·Journal of Bioenergetics and Biomembranes·Masatoshi Murai, Hideto Miyoshi
Sep 12, 2012·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Hyoung Youn LeeIn Seok Jeong
Dec 3, 2015·Biochimica Et Biophysica Acta·Masatoshi Murai, Hideto Miyoshi
Feb 28, 2016·Biochimica Et Biophysica Acta·Christophe WirthVolker Zickermann
May 27, 2015·Biochimica Et Biophysica Acta·Anna StepanovaAlexander Galkin
Mar 4, 2015·Bioscience, Biotechnology, and Biochemistry·Masatoshi MuraiHideto Miyoshi
Oct 16, 2015·Journal of Economic Entomology·L LopezJ R Bloomquist
Apr 15, 2015·Biochimica Et Biophysica Acta·Madhavan NarayananEiko Nakamaru-Ogiso
Sep 18, 2012·The International Journal of Biochemistry & Cell Biology·Marina Verkhovskaya, Dmitry A Bloch
Apr 10, 2018·Clinical Nuclear Medicine·Kyeong Joon KimSang Eun Kim
Aug 3, 2012·The Journal of Biological Chemistry·Philippa G Roberts, Judy Hirst
Apr 15, 2020·Pesticide Biochemistry and Physiology·Elaheh Shafiei AlavijehWannes Dermauw
Apr 17, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Outi Haapanen, Vivek Sharma
Apr 8, 2015·Biochemistry·Masatoshi MuraiHideto Miyoshi
Aug 3, 2021·Antimicrobial Agents and Chemotherapy·Keith Kiplangat TalaamShinjiro Hamano

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