Fentanyl Induces Cerebellar Internal Granular Cell Layer Apoptosis in Healthy Newborn Pigs

Frontiers in Neurology
Hemmen SabirMarianne Thoresen

Abstract

Opioids like fentanyl are regularly used in neonates for analgesia and sedation. So far, they have been reported to be safe and eligible to use. The cerebellum has become a focus of neurodevelopmental research within the last years, as it is known to play an important role in long-lasting motor, cognitive, and other behavioral changes. The cerebellar cortex is of major importance in the coordinative role of the cerebellum and highly vulnerable to injury and impaired growth. This study was performed to evaluate the apoptotic effect of intravenous fentanyl infusion on the cerebellum in healthy newborn pigs. Thirteen healthy pigs (<median 12 h old) were randomized into (1) 24 h of intravenous fentanyl at normothermia (NTFe, n = 6) or (2) non-ventilated controls at normothermia (NTCTR, n = 7). Cerebellar sections were morphologically assessed after staining with hematoxylin-eosin. In addition, paired sections were immuno-stained for cell death [Cleaved caspase-3 and terminal deoxynucleotidyl transferase-mediated deoxyuridine-triphosphate nick-end labeling (TUNEL)], and positive cells were counted in defined areas of the internal granular cell layer. In total, cells in three cerebellar gyri were counted. We found that there was an i...Continue Reading

References

Mar 1, 1979·Early Human Development·J Dobbing, J Sands
Apr 6, 1981·Brain Research·J A Schulman, F E Bloom
Jan 1, 1993·Brain Research Bulletin·J V Seatriz, R P Hammer
Nov 3, 1998·Archives of Disease in Childhood. Fetal and Neonatal Edition·R MacGregorM Levene
Apr 9, 1999·Archives of Disease in Childhood. Fetal and Neonatal Edition·P LagoF Zacchello
Jan 1, 1954·The Anatomical Record·O LARSELL
May 26, 2004·Progress in Neurobiology·Constantino Sotelo
Aug 20, 2004·Pharmacological Reviews·Irmgard Tegeder, Gerd Geisslinger
Mar 3, 2005·Pediatrics·Catherine LimperopoulosAdré J du Plessis
Mar 19, 2005·Pediatric Research·Danilyn M AngelesStephen Ashwal
Jul 30, 2005·Neuromolecular Medicine·Graeme Milligan
Jan 5, 2006·Cellular and Molecular Neurobiology·Marong FangDavid T Yew
Aug 6, 2008·Archives of Pediatrics & Adolescent Medicine·Jean-Christophe RozéGérard Bréart
Aug 30, 2008·British Journal of Anaesthesia·K J S AnandUNKNOWN NEOPAIN Trial Investigators Group
Jan 22, 2010·Pediatric Research·Xavier DurrmeyerPeter C Rimensberger
May 29, 2010·Frontiers in Cellular Neuroscience·Sergio SolinasEgidio D'Angelo
Apr 6, 2011·Archives of Pediatrics & Adolescent Medicine·Susan E JacobsUNKNOWN Infant Cooling Evaluation Collaboration
Apr 27, 2011·Annals of Neurology·Frances J NorthingtonLee J Martin
Dec 14, 2011·Intensive Care Medicine·Elavazhagan ChakkarapaniJohn Dingley
Apr 25, 2012·Neurology Research International·Valerie BiranDonna M Ferriero
Apr 18, 2013·Anesthesiology·Hemmen SabirMarianne Thoresen
Sep 23, 2014·The Cerebellum·Marie Brossard-RacineCatherine Limperopoulos
Jul 18, 2015·Neurobiology of Disease·Xiangning Jiang, Jeannette Nardelli
Sep 16, 2015·The Annals of Pharmacotherapy·Christopher McPhersonTerrie E Inder
Dec 19, 2016·The Journal of Pediatrics·Dawn Gano, Donna M Ferriero
Sep 25, 2017·Archives of Disease in Childhood. Fetal and Neonatal Edition·Vânia OliveiraSudhin Thayyil

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Citations

Feb 27, 2019·Therapeutic Hypothermia and Temperature Management·Natasha LiowSudhin Thayyil
Apr 9, 2021·Peptides·Richard J Bodnar
Jun 29, 2021·Frontiers in Systems Neuroscience·Giulia SpotoGabriella Di Rosa
Jul 4, 2021·Seminars in Fetal & Neonatal Medicine·Christopher McPhersonUNKNOWN Newborn Brain Society Guidelines and Publications Committee
Jul 27, 2021·EClinicalMedicine·Julia K GundersenMarianne Thoresen

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Methods Mentioned

BETA
sedation

Software Mentioned

SPSS

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis