Fermentation of acid-pretreated corn stover to ethanol without detoxification using Pichia stipitis

Applied Biochemistry and Biotechnology
Frank K AgbogboKevin S Wenger

Abstract

In this work, the effect of adaptation on P. stipitis fermentation using acid-pretreated corn stover hydrolyzates without detoxification was examined. Two different types of adaptation were employed, liquid hydrolyzate and solid state agar adaptation. Fermentation of 12.5% total solids undetoxified acid-pretreated corn stover was performed in shake flasks at different rotation speeds. At low rotation speed (100 rpm), both liquid hydrolyzate and solid agar adaptation highly improved the sugar consumption rate as well as ethanol production rate compared to the wild-type strains. The fermentation rate was higher for solid agar-adapted strains compared to liquid hydrolyzate-adapted strains. At a higher rotation speed (150 rpm), there was a faster sugar consumption and ethanol production for both the liquid-adapted and the wild-type strains. However, improvements in the fermentation rate between the liquid-adapted and wild strains were less pronounced at the high rotation speed.

References

Jul 23, 1997·Journal of Biotechnology·J Lee
Jan 15, 1999·Critical Reviews in Biotechnology·P Chandrakant, V S Bisaria
Feb 13, 2007·Applied Microbiology and Biotechnology·Bärbel Hahn-HägerdalMarie F Gorwa-Grauslund
May 15, 2008·Applied Biochemistry and Biotechnology·Frank K AgbogboThomas W Jeffries

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Citations

Apr 24, 2008·Biotechnology Letters·Frank K Agbogbo, Guillermo Coward-Kelly
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph B Binder, Ronald T Raines
Feb 18, 2016·Bioprocess and Biosystems Engineering·Nilesh Kumar SharmaSachin Kumar

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