PMID: 9170247Apr 1, 1997Paper

Fermentation of biomass-derived glucuronic acid by pet expressing recombinants of E. coli B

Applied Biochemistry and Biotechnology
H G Lawford, J D Rousseau

Abstract

The economics of large-scale production of fuel ethanol from biomass and wastes requires the efficient utilization of all the sugars derived from the hydrolysis of the heteropolymeric hemicellulose component of lignocellulosic feedstocks. Glucuronic and 4-O-methyl-glucuronic acids are major side chains in xylans of the grasses and hardwoods that have been targeted as potential feedstocks for the production of cellulosic ethanol. The amount of these acids is similar to that of arabinose, which is now being viewed as another potential substrate in the production of biomass-derived ethanol. This study compared the end-product distribution associated with the fermentation of D-glucose (Glc) and D-glucuronic acid (GlcUA) (as sole carbon and energy sources) by Escherichia coli B (ATCC 11303) and two different ethanologenic recombinants--a strain in which pet expression was via a multicopy plasmid (pLOI297) and a chromosomally integrated construct, strain KO11. pH-stat batch fermentations were conducted using a modified LB medium with 2% (w/v) Glc or GlcUA with the set-point for pH control at either 6.3 or 7.0. The nontransformed host culture produced only lactic acid from glucose, but fermentation of GlcUA yielded a mixture of ethano...Continue Reading

References

Jan 1, 1992·Applied Biochemistry and Biotechnology·H G Lawford, J D Rousseau
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Citations

Sep 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jennifer C MortimerPaul Dupree
Dec 1, 2004·EcoSal Plus·R Gary Sawers, David P Clark

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