Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: simplex optimisation of media supplements

Applied Microbiology and Biotechnology
S GoughR Marchant

Abstract

The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentration of the supplements were 0.576 g1(-1) magnesium sulphate, 0.288 g1(-1) potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.

Citations

Apr 23, 2008·Applied Microbiology and Biotechnology·Gustavo Graciano FonsecaAndreas K Gombert
Apr 17, 2007·Journal of Biotechnology·Jiong HongHisanori Tamaki
Jun 6, 2008·Bioresource Technology·Mirbella Cáceres-FarfánLuis Barahona-Pérez
Nov 30, 2019·Applied Microbiology and Biotechnology·Mochamad NurcholisMamoru Yamada

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