Abstract
A simple and cost-effective biochemical conversion process consisting of hydrothermal treatment, enzymatic hydrolysis and fermentation of pretreated whole slurry (PWS) was developed for producing l-lactic acid (L-LA) from oil palm trunk (OPT). When OPT was hydrothermally treated at optimal condition capable of achieving maximum yield of hemicellulosic sugars after enzymatic hydrolysis, the enzymatic digestibility of the PWS afforded a yield of 81.4% of the theoretical glucose yield (TGY). However, glucose yield from washed pretreated solid (WPS) was only 43.5% of TGY. The use of two hydrolysates from PWS and WPS for fermentation by Lactobacillus paracasei engineered to selectively produce L-LA afforded yields of 89.5% and 45.8% of the theoretical LA yield (TLY), respectively. This study confirmed the inevitable extensive sugar loss during washing of pretreated slurry due to loss of soluble starch. Alternatively, the proposed design process is considered suitable for converting OPT to L-LA without such starch loss.
References
Mar 10, 2004·Biotechnology Letters·Young-Jung WeeHwa-Won Ryu
Oct 20, 2005·Journal of Bioscience and Bioengineering·H K SreenathR J Straub
May 5, 2006·Applied and Environmental Microbiology·Milind A PatelK T Shanmugam
Oct 31, 2006·Applied and Environmental Microbiology·Marja IlménMerja Penttilä
Jan 17, 2007·Applied Microbiology and Biotechnology·Rojan P JohnAshok Pandey
Feb 15, 2007·Applied Biochemistry and Biotechnology·A B MoldesJ M Domínguez
Jun 2, 2007·Biotechnology Letters·Buenaventurada P Calabia, Yutaka Tokiwa
Feb 6, 2008·Applied Microbiology and Biotechnology·Ronald H W MaasRuud A Weusthuis
Sep 22, 2009·Bioresource Technology·Pattana LaopaiboonLakkana Laopaiboon
Nov 26, 2009·Applied Biochemistry and Biotechnology·Wei GuoShulin Chen
Jan 9, 2010·Bioresource Technology·Prasad Kaparaju, Claus Felby
Jun 16, 2010·Journal of Bioscience and Bioengineering·Akihiko KosugiYutaka Mori
Jul 16, 2010·Bioresource Technology·Limin WangYanhe Ma
Jul 7, 2011·Journal of Biotechnology·Mohamed Ali Abdel-RahmanKenji Sonomoto
Oct 2, 2012·Bioresource Technology·Panida PrawitwongYutaka Mori
Apr 25, 2013·Bioresource Technology·Chan-Duck JungKyung-Sik Hong
Sep 24, 2013·Biotechnology for Biofuels·Riin KontPriit Väljamäe
Citations
Feb 27, 2016·Bioresource Technology·Jae Hoon Lee, Eun Yeol Lee
Oct 12, 2015·Bioresource Technology·Gang LiuJie Bao
Mar 10, 2016·Bioresource Technology·Shashi Kant BhatiaYung-Hun Yang
Apr 5, 2016·Waste Management·Jialing TangYuyou Li
Jun 5, 2016·Microbial Cell Factories·Hee Su KimSi Jae Park
Mar 30, 2017·Applied Biochemistry and Biotechnology·Xingxuan ChenMin-Tian Gao
Aug 20, 2019·Regenerative Engineering and Translational Medicine·Kenneth S OgueriCato T Laurencin
Oct 2, 2020·Microorganisms·Zhenting ZhangShumiao Zhao