Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats

The ISME Journal
Xiyang DongRainer U Meckenstock

Abstract

Spirochaetes are frequently detected in anoxic hydrocarbon- and organohalide-polluted groundwater, but their role in such ecosystems has remained unclear. To address this, we studied a sulfate-reducing, naphthalene-degrading enrichment culture, mainly comprising the sulfate reducer Desulfobacterium N47 and the rod-shaped Spirochete Rectinema cohabitans HM. Genome sequencing and proteome analysis suggested that the Spirochete is an obligate fermenter that catabolizes proteins and carbohydrates, resulting in acetate, ethanol, and molecular hydrogen (H2) production. Physiological experiments inferred that hydrogen is an important link between the two bacteria in the enrichment culture, with H2 derived from fermentation by R. cohabitans used as reductant for sulfate reduction by Desulfobacterium N47. Differential proteomics and physiological experiments showed that R. cohabitans utilizes biomass (proteins and carbohydrates) released from dead cells of Desulfobacterium N47. Further comparative and community genome analyses indicated that other Rectinema phylotypes are widespread in contaminated environments and may perform a hydrogenogenic fermentative lifestyle similar to R. cohabitans. Together, these findings indicate that enviro...Continue Reading

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Citations

Jul 28, 2018·Environmental Microbiology·Anne M HimmelbergRainer U Meckenstock
Jan 20, 2019·Applied and Environmental Microbiology·Sarah Piché-Choquette, Philippe Constant
Jun 28, 2019·The ISME Journal·Chris GreeningRoderick I Mackie
Mar 13, 2019·Nature Microbiology·Adam J KesslerChris Greening
Jul 14, 2020·Frontiers in Microbiology·Elena TamburiniFrancesco Vitali
May 28, 2021·Environmental Science & Technology·Courtney R A TothElizabeth A Edwards
Jun 26, 2021·Environmental Science & Technology·Steven D MametSteven D Siciliano
Jul 22, 2021·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Anumeha SahaPinaki Sar
Jul 29, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Raul Matias CezarRudimar Molin
Aug 1, 2021·Nature Communications·Janina RahlffAlexander J Probst
Nov 16, 2021·Frontiers in Microbiology·Federico A VignaleMaría E Farías
Jan 31, 2022·The Science of the Total Environment·Soraya ZahediMaite Pijuan

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Datasets Mentioned

BETA
PXD005624

Methods Mentioned

BETA
genome-based predictions

Software Mentioned

dbCAN
HydDB
MaGe )
MEROPS
Magnifying Genome (
MicroScope
MEGA7
MaGe

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