Fermented Antrodia cinnamomea extract protects rat PC12 cells from serum deprivation-induced apoptosis: the role of the MAPK family

Journal of Agricultural and Food Chemistry
Mei-Kuang LuNai-Kuei Huang

Abstract

Antrodia cinnamomea (formerly A. camphorata) has recently and commercially been used in the formulation of nutraceuticals and functional foods in Taiwan. Because of its diverse properties, the neuroprotective effect was investigated using a fermented A. cinnamomea extract in this study. Serum deprivation-induced apoptosis in neuronal-like pheochromocytoma (PC12) cells was used as a cell stress model, and it was found that A. cinnamomea was effective in preventing serum-deprived apoptosis according to results of an MTT assay and Hoechst staining. Serum deprivation resulted in decreased phosphorylation of extracellular signal-regulated kinase (ERK) and increased phosphorylations of c-Jun NH2-terminal kinase (JNK) and p38, of the family of mitogen-activated protein kinases (MAPKs); however, A. cinnamomea reversed these phenomena, supporting the antagonistic effects between ERK and JNK-p38 in regulating cell survival. The previously identified active component of A. cinnamomea, adenosine (ADO), also exerted the same effects as A. cinnamomea in preventing apoptosis and regulating phosphorylations of MAPKs. Although an inhibitor of the ERK upstream activator blocked A. cinnamomea-induced ERK phosphorylations, it failed to block the p...Continue Reading

Citations

Apr 9, 2013·Pharmacology & Therapeutics·Mei-Chin LuYang-Chang Wu
Aug 19, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Madamanchi Geethangili, Yew-Min Tzeng
Apr 7, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yu-Jen ChenTun-Tschu Chang
Dec 9, 2008·Journal of Ethnopharmacology·Zong-Hua AoWen-Fang Dou
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Nov 11, 2017·Critical Reviews in Biotechnology·Chia-Wei PhanVikineswary Sabaratnam
Oct 8, 2019·3 Biotech·Adhiraj Dasgupta, Krishnendu Acharya

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis