Ferritin nanoparticles for improved self-renewal and differentiation of human neural stem cells

Biomaterials Research
Jung Seung LeeSeung-Woo Cho

Abstract

Biomaterials that promote the self-renewal ability and differentiation capacity of neural stem cells (NSCs) are desirable for improving stem cell therapy to treat neurodegenerative diseases. Incorporation of micro- and nanoparticles into stem cell culture has gained great attention for the control of stem cell behaviors, including proliferation and differentiation. In this study, ferritin, an iron-containing natural protein nanoparticle, was applied as a biomaterial to improve the self-renewal and differentiation of NSCs and neural progenitor cells (NPCs). Ferritin nanoparticles were added to NSC or NPC culture during cell growth, allowing for incorporation of ferritin nanoparticles during neurosphere formation. Compared to neurospheres without ferritin treatment, neurospheres with ferritin nanoparticles showed significantly promoted self-renewal and cell-cell interactions. When spontaneous differentiation of neurospheres was induced during culture without mitogenic factors, neuronal differentiation was enhanced in the ferritin-treated neurospheres. In conclusion, we found that natural nanoparticles can be used to improve the self-renewal ability and differentiation potential of NSCs and NPCs, which can be applied in neural tis...Continue Reading

References

Aug 1, 1994·Journal of Neurochemistry·A J Roskams, J R Connor
Jun 1, 1993·Nutrition Reviews·J L BeardB C Jones
May 4, 2002·Nature Reviews. Neuroscience·Ferdinando Rossi, Elena Cattaneo
Apr 24, 2004·Annals of the New York Academy of Sciences·Torben Moos, Evan H Morgan
Jun 9, 2006·Nature Reviews. Neuroscience·Gianvito Martino, Stefano Pluchino
Oct 18, 2006·Journal of Neuroscience Research·Hideki MoriYonehiro Kanemura
Apr 5, 2007·Nature Protocols·Stefanie Kaech, Gary Banker
Jul 3, 2009·Small·Aihui MahamYuehe Lin
May 10, 2011·Acta Biomaterialia·Kentaro Hayashi, Yasuhiko Tabata
Apr 20, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David L SchonbergDana M McTigue
Feb 8, 2014·Ageing Research Reviews·Amy ReeveDoug Turnbull
Jul 19, 2014·Tissue Engineering. Part C, Methods·David LuWei-Chun Chin
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiangXiyun Yan
Mar 17, 2015·Stem Cell Research·Stella Alimperti, Stelios T Andreadis
Jun 21, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Hye In SeoBong Geun Chung

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Citations

Jan 1, 2020·International Journal of Molecular Sciences·Yeon-Woo ChoTae-Hyung Kim
Jun 27, 2019·Stem Cell Research & Therapy·Anna AndrzejewskaBarbara Lukomska
Sep 29, 2019·Biomacromolecules·Madhumita PatelByeongmoon Jeong

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Methods Mentioned

BETA
PCR

Software Mentioned

Image J
GraphPad Prism

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