Ferritin protein nanocage ion channels: gating by N-terminal extensions.

The Journal of Biological Chemistry
Takehiko ToshaElizabeth C Theil

Abstract

Ferritin protein nanocages, self-assembled from four-α-helix bundle subunits, use Fe(2+) and oxygen to synthesize encapsulated, ferric oxide minerals. Ferritin minerals are iron concentrates stored for cell growth. Ferritins are also antioxidants, scavenging Fenton chemistry reactants. Channels for iron entry and exit consist of helical hairpin segments surrounding the 3-fold symmetry axes of the ferritin nanocages. We now report structural differences caused by amino acid substitutions in the Fe(2+) ion entry and exit channels and at the cytoplasmic pores, from high resolution (1.3-1.8 Å) protein crystal structures of the eukaryotic model ferritin, frog M. Mutations that eliminate conserved ionic or hydrophobic interactions between Arg-72 and Asp-122 and between Leu-110 and Leu-134 increase flexibility in the ion channels, cytoplasmic pores, and/or the N-terminal extensions of the helix bundles. Decreased ion binding in the channels and changes in ordered water are also observed. Protein structural changes coincide with increased Fe(2+) exit from dissolved, ferric minerals inside ferritin protein cages; Fe(2+) exit from ferritin cages depends on a complex, surface-limited process to reduce and dissolve the ferric mineral. High...Continue Reading

References

Jan 19, 1978·Nature·S H BanyardP M Harrison
Jan 1, 1990·Annual Review of Nutrition·R D Baynes, T H Bothwell
May 23, 1996·Nature·R W HooftE E Abola
Jul 21, 1998·The Journal of Biological Chemistry·H TakagiE C Theil
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C R KissingerD B Fogel
Aug 10, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Y HaN M Allewell
Feb 1, 2000·The Journal of Biological Chemistry·C FerreiraC Beaumont
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
Mar 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng LiuElizabeth C Theil
Apr 5, 2003·Protein Engineering·Zheng YuanZhi-Xin Wang
Jan 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·James Holton, Tom Alber
Aug 10, 2004·Acta Crystallographica. Section D, Biological Crystallography·Y HaN M Allewell
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 3, 2005·Protein Science : a Publication of the Protein Society·Krisna C Duong-LyKathleen P Howard
Mar 16, 2005·Accounts of Chemical Research·Xiaofeng Liu, Elizabeth C Theil
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Andrew G W Leslie
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Nov 11, 2006·Science·Francis I ValiyaveetilRoderick Mackinnon
Sep 6, 2007·The Journal of Biological Chemistry·Xiaofeng S LiuElizabeth C Theil
Nov 23, 2007·Journal of Molecular Biology·Lee MakowskiRobert F Fischetti
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takehiko ToshaElizabeth C Theil
Jan 15, 2009·Journal of the American Chemical Society·Conggang Li, Gary J Pielak
Mar 26, 2009·Annual Review of Biochemistry·Gary J Doherty, Harvey T McMahon
Oct 15, 2009·Chemical Reviews·Elizabeth C Theil, Dixie J Goss
Nov 26, 2009·Seminars in Cell & Developmental Biology·Richard Lundmark, Sven R Carlsson
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Paola TuranoIvano Bertini
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons
Apr 15, 2010·Biochimica Et Biophysica Acta·Nick E Le BrunGeoffrey R Moore
Jul 7, 2010·Cell·Matthias W HentzeClara Camaschella
Sep 28, 2010·Journal of the American Chemical Society·Takehiko ToshaElizabeth C Theil
Oct 29, 2010·Journal of Cell Science·Kiros HailemariamYoshiaki Tsuji
Dec 15, 2010·Biology of the Cell·Graça SoveralMaria C Loureiro-Dias
Jan 20, 2011·Biophysical Journal·Avia Rosenhouse-DantskerIrena Levitan
Mar 9, 2011·The Journal of Biological Chemistry·Shimrit OzNathan Dascal
Mar 15, 2011·Biochemical and Biophysical Research Communications·Atsushi InanobeYoshihisa Kurachi
May 14, 2011·Metallomics : Integrated Biometal Science·Pierre CornelisTiffany Vinckx
May 20, 2011·The Journal of Biological Chemistry·Suranjana HaldarElizabeth C Theil

❮ Previous
Next ❯

Citations

Nov 22, 2012·Biochemistry·Huan RuiWonpil Im
Oct 25, 2012·Inorganic Chemistry·Takehiko ToshaElizabeth C Theil
Oct 10, 2013·Inorganic Chemistry·Elizabeth C Theil
Oct 25, 2013·Nutrients·Maria C Linder
Mar 25, 2014·The Journal of Chemical Physics·Anatoly M RuvinskyMario Rivera
Feb 8, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elizabeth C TheilCaterina Bernacchioni
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rabindra K Behera, Elizabeth C Theil
Jul 24, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rabindra K BeheraElizabeth C Theil
Mar 9, 2013·Coordination Chemistry Reviews·Elizabeth C TheilTakehiko Tosha
Jan 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu ZhangBrendan P Orner
Feb 20, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera
Feb 28, 2014·The Journal of Biological Chemistry·Sunanda Margrett WilliamsDipankar Chatterji
Apr 30, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mohamad F AslamDora I A Pereira
Apr 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Cecilia PozziStefano Mangani
Sep 1, 2016·Oncotarget·Laura ContiSimonetta Geninatti Crich
Oct 29, 2014·Chemical Communications : Chem Comm·Unai CarmonaMato Knez
Apr 19, 2017·Metallomics : Integrated Biometal Science·Sunanda Margrett Williams, Dipankar Chatterji
Feb 19, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Prashanth Kumar KoochanaRabindra K Behera
Apr 27, 2021·European Biophysics Journal : EBJ·João P JacintoAlice S Pereira
Nov 25, 2014·Chemical Reviews·Kourosh Honarmand EbrahimiWilfred R Hagen
May 1, 2020·Chemical Research in Toxicology·Uschi M GrahamDavid A Bennett
Feb 18, 2014·The Journal of Physical Chemistry. a·Rozita LaghaeiRob D Coalson
Aug 31, 2017·Journal of Chemical Information and Modeling·Davide SalaAntonio Rosato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.