Ferrodrop Dose-Optimized Digital Quantification of Biomolecules in Low-Volume Samples

Analytical Chemistry
Soroush KahkeshaniDino Di Carlo

Abstract

We present an approach to estimate the concentration of a biomolecule in a solution by sampling several nanoliter-scale volumes and determining if the volumes contain any biomolecules. In this method, varying volume fractions (nanoliter-scale) of a sample of nucleic acids are introduced to an array of uniform volume reaction wells (100 μL), which are then fluorescently imaged to determine if signal is above a threshold after nucleic acid amplification, all without complex instrumentation. The nanoliter volumes are generated and introduced using the simple positioning of a permanent magnet, and imaging is performed with a cellphone-based fluorescence detection scheme, both methods suitable for limited-resource settings. We use the length of time a magnetic field is applied to generate a calibrated number of nanoliter ferrodrops of sample mixed with ferrofluid at a step emulsification microfluidic junction. Each dose of ferrodrops is then transferred into larger microliter scale reaction wells on chip through a simple shift of the external magnet. Nucleic acid amplification is achieved using loop-mediated isothermal amplification (LAMP). By repeating each nanoliter dosage a number of times to calculate the probability of a positi...Continue Reading

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Citations

Oct 7, 2020·Science Robotics·Wenzhuo YuSam Emaminejad
Mar 28, 2021·Biosensors & Bioelectronics·Juhwan ParkJe-Kyun Park
Nov 19, 2021·Physical Chemistry Chemical Physics : PCCP·Dongliang LiQiang Liao

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