Ferroportin 1 but not hephaestin contributes to iron accumulation in a cell model of Parkinson's disease

Free Radical Biology & Medicine
Ning SongJun-Xia Xie

Abstract

Iron-induced oxidative stress is thought to play a crucial role in the pathogenesis of Parkinson's disease (PD). Based on our previous in vivo experiments showing that down-regulation of the iron transporters ferroportin 1 (FP1) and hephaestin (HP) might account for the nigral iron accumulation in 6-hydroxydopamine (6-OHDA)-lesioned animal models, in this study we investigated whether FP1 and HP were involved in cellular iron accumulation and the underlying mechanisms in a cell model of PD. The findings showed that 6-OHDA induced FP1 and HP down-regulation, followed by decreased iron efflux and iron accumulation in primary ventral mesencephalic neurons and MES23.5 dopaminergic cells. Silencing of FP1 but not HP led to increased iron levels and aggravated reactive oxygen species generation in MES23.5 cells. Under iron-overloaded conditions, FP1 showed dose-dependent up-regulation, whereas HP showed no response, indicating their down-regulation was not due to the increased intracellular iron content. In 6-OHDA-treated cells, both iron-regulatory protein (IRP) 1 and IRP2 were up-regulated, and silencing of IRPs in MES23.5 cells dramatically blocked 6-OHDA-induced FP1 down-regulation and reversed HP down-regulation. These results s...Continue Reading

Citations

Dec 17, 2010·Journal of Neural Transmission·Xuping LiWeidong Le
Feb 4, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pabla AguirreMarco T Núñez
Feb 10, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Marco T NúñezJulio Salazar
Jun 19, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hao-Yun ZhangJun-Xia Xie
Jan 23, 2016·Journal of Neural Transmission·Kai Li, Heinz Reichmann
Jan 16, 2016·Parkinson's Disease·Dan ChenManju B Reddy
Oct 16, 2015·Molecular Neurobiology·Giorgio BiasiottoIsabella Zanella
Apr 4, 2016·Molecular Neurobiology·Hong JiangJunxia Xie
Aug 8, 2014·Frontiers in Aging Neuroscience·David B Lovejoy, Gilles J Guillemin
Sep 19, 2014·Lancet Neurology·Roberta J WardLuigi Zecca
Aug 23, 2018·Journal of Cellular Physiology·Manman XuJun Wang
Feb 9, 2018·Journal of Translational Medicine·Driton Vela
Jun 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Huamin XuJunxia Xie
Jan 17, 2013·Metallomics : Integrated Biometal Science·Dominic J HareDavid I Finkelstein
Sep 4, 2020·Experimental Biology and Medicine·Santosh R D'Mello, Mark C Kindy
Oct 31, 2018·Frontiers in Neuroscience·Driton Vela
Nov 3, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Qing LinWei Wang
Feb 4, 2021·The International Journal of Neuroscience·Thomas M Berry, Ahmed A Moustafa
Jul 30, 2020·Progress in Neurobiology·Laura Mahoney-SánchezJean-Christophe Devedjian
Dec 28, 2018·ACS Chemical Neuroscience·Lei-Lei ChenJunxia Xie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.