Ferrous and ferric differentially deteriorate proliferation and differentiation of osteoblast-like UMR-106 cells

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Kornkamon LertsuwanNarattaphol Charoenphandhu

Abstract

The association between iron overload and osteoporosis has been found in many diseases, such as hemochromatosis, β-thalassemia and sickle cell anemia with multiple blood transfusion. One of the contributing factors is iron toxicity to osteoblasts. Some studies showed the negative effects of iron on osteoblasts; however, the effects of two biological available iron species, i.e., ferric and ferrous, on osteoblasts are elusive. Since most intracellular ionized iron is ferric, osteoblasts was hypothesized to be more responsive to ferric iron. Herein, ferric ammonium citrate (FAC) and ferrous ammonium sulfate (FAS) were used as ferric and ferrous donors. Our results showed that both iron species suppressed cell survival and proliferation. Both also induced osteoblast cell death consistent with the higher levels of cleaved caspase 3 and caspase 7 in osteoblasts, indicating that iron induced osteoblast apoptosis. Iron treatments led to the elevated intracellular iron in osteoblasts as determined by atomic absorption spectrophotometry, thereby leading to a decreased expression of genes for cellular iron import and increased expression of genes for cellular iron export. Effects of FAC and FAS on osteoblast differentiation were determin...Continue Reading

References

Dec 1, 1995·Toxicology Letters·C C Winterbourn
May 16, 2003·Nature·William J BoyleDavid L Lacey
Sep 24, 2004·International Review of Cytology·Vidosava B Djordjević
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Tomas Ganz
Mar 11, 2008·Antioxidants & Redox Signaling·Elizabeth L MacKenzieYoshiaki Tsuji
Apr 15, 2008·Archives of Biochemistry and Biophysics·Koichi Matsuo, Naoko Irie
Nov 26, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Aude BressenotFrançois Plénat
Mar 26, 2009·Clinical Oral Investigations·Peter Proff, Piero Römer
Sep 9, 2009·Toxicology Letters·Kanako Yamasaki, Hiromi Hagiwara
Oct 14, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Abolfazl ZarjouJózsef Balla
Nov 7, 2009·Methods in Enzymology·James E Noble, Marc J A Bailey
May 27, 2010·The Journal of Biological Chemistry·Liza J Raggatt, Nicola C Partridge
Jan 15, 2011·International Journal of Alzheimer's Disease·Gabriela A SalvadorNorma M Giusto
Jan 22, 2013·Biological Trace Element Research·Yin-Feng HeYou-Jia Xu
Aug 15, 2014·Experimental and Therapeutic Medicine·Guo-Yang ZhaoYou-Jia Xu
Nov 30, 2015·Calcified Tissue International·Wenjie XieWilly Hofstetter
Sep 8, 2016·Journal of Biomedical Materials Research. Part a·Dongmei ZhangYuncang Li
Mar 9, 2017·Frontiers in Pharmacology·Viktória Jeney
Apr 4, 2017·International Journal of Medical Sciences·Fei LiuMao-Wei Yang

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Citations

Jun 19, 2020·Journal of Diabetes and Metabolic Disorders·Mohammad Reza Mohajeri-TehraniZohreh Hamidi
May 11, 2021·Transplantation and Cellular Therapy·Alessandro IsidoriFrancesca Vinchi
Aug 8, 2021·Journal of Clinical Medicine·Leiyu ShiShin-Yi Tsai
Oct 14, 2021·PloS One·Supathra PhoaubonNarattaphol Charoenphandhu

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Methods Mentioned

BETA
Assay
scraping
protein assay
flame atomic absorption spectroscopy

Software Mentioned

cellSens

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