Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide - a comparative study

The FEBS Journal
Alessandro BolliPaolo Ascenzi

Abstract

Campylobacter jejuni hosts two hemoglobins (Hbs). The Camplylobacter jejuni single-domain Hb (called Cgb) is homologous to the globin domain of flavohemoglobin, and it has been proposed to protect the bacterium against nitrosative stress. The second Hb is called Ctb (hereafter Cj-trHbP), belongs to truncated Hb group III, and has been hypothesized to be involved in O(2) chemistry. Here, the kinetics and thermodynamics of cyanide binding to ferric and ferrous Cj-trHbP [Cj-trHbP(III) and Cj-trHbP(II), respectively] are reported and analyzed in parallel with those of related heme proteins, with particular reference to those from Mycobacterium tuberculosis. The affinity of cyanide for Cj-trHbP(II) is higher than that reported for any known (in)vertebrate globin by more than three orders of magnitude (K = 1.2 x 10(-6) m). This can be fully attributed to the highest (ever observed for a ferrous Hb) cyanide-binding association rate constant (k(on) = 3.3 x 10(3) m(-1).s(-1)), even though the binding process displays a rate-limiting step (k(max) = 9.1 s(-1)). Cj-trHbP(III) shows a very high affinity for cyanide (L = 5.8 x 10(-9) m); however, cyanide association kinetics are independent of cyanide concentration, displaying a rate-limitin...Continue Reading

References

Mar 1, 1971·The Biochemical Journal·C PhelpsM Brunori
Apr 13, 1994·Biochimica Et Biophysica Acta·G AntoniniM Brunori
Jan 1, 1997·Progress in Biophysics and Molecular Biology·M BolognesiP Ascenzi
Mar 19, 1999·Emerging Infectious Diseases·S F AltekruseD L Swerdlow
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M CoutureM Guertin
Nov 7, 2001·The Journal of Biological Chemistry·Jonathan B WittenbergMichel Guertin
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mario MilaniMartino Bolognesi
Oct 17, 2003·Advances in Microbial Physiology·Guanghui WuRobert K Poole
Mar 16, 2004·The Journal of Biological Chemistry·Mario MilaniMartino Bolognesi
May 5, 2004·Biochemistry·Mario MilaniPaolo Ascenzi
Apr 23, 2005·Veterinary Research·John E MoorePaul Whyte
Feb 14, 2006·Journal of Molecular Evolution·David A Vuletich, Juliette T J Lecomte
Apr 8, 2006·BMC Evolutionary Biology·Serge N VinogradovJacques R Vanfleteren
Oct 7, 2006·The Journal of Biological Chemistry·Marco NardiniMartino Bolognesi
May 29, 2007·Gene·Paolo AscenziPaolo Visca
Aug 19, 2007·Nature Reviews. Microbiology·Kathryn T YoungVictor J Dirita
Nov 1, 2006·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Enrique Claro

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Citations

Oct 12, 2014·Journal of Inorganic Biochemistry·Selena L RiceJuliette T J Lecomte
Oct 17, 2012·Biochemical and Biophysical Research Communications·Paolo AscenziFabio Polticelli
Feb 2, 2010·Biochemical and Biophysical Research Communications·Paolo AscenziMassimo Coletta
Mar 30, 2011·IUBMB Life·Henry J NothnagelJuliette T J Lecomte
May 28, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Jun 25, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Sep 4, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo Ascenzi, Alessandra Pesce
Mar 9, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Apr 2, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Nov 17, 2019·Journal of Inorganic Biochemistry·Paolo AscenziMassimo Coletta
Jul 15, 2020·Molecular Aspects of Medicine·Alessandra di MasiPaolo Ascenzi
Feb 7, 2018·Biochemistry·Zachary GeeraertsGudrun S Lukat-Rodgers

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