Abstract
Oxidative damage caused by the ferryl hemoglobin is one of the major clinical adverse reactions of hemoglobin-based oxygen carriers (HBOCs), while the production of reactive oxygen species in a pathological state can oxidize hemoglobin (HbFe2+ ) to ferryl Hb, which can then enter the pseudoperoxidase cycle, making hemoglobin highly toxic. In this study, we found that ferrous hemoglobin and polymerized porcine hemoglobin (one of the HBOCs) have the peroxidase activity different from the pseudoperoxidase activity of ferric hemoglobin. Ferrous hemoglobin can catalyze the reaction of tyrosine (Tyr) with hydrogen peroxide. In addition, the results also indicated that ferrous hemoglobin and pPolyHb have a strong inhibitory effect on the pseudoperoxidase activity of ferric hemoglobin. Therefore, hydrogen peroxide was consumed in a large amount, which greatly prevented hemoglobin from becoming oxidized and entering the pseudoperoxidase cycle, thus inhibiting ferryl Hb toxicity. We further cultured human umbilical vein endothelial cells and monitored cell morphology, viability, cell cycle, apoptosis, lactate dehydrogenase (LDH) release, and malondialdehydes (MDAs) formation when incubated with H2 O2 , Tyr, and HbFe2+ . HbFe2+ and pPolyH...Continue Reading
References
Jun 1, 1995·Molecular Medicine Today·A I Alayash, R E Cashon
Jan 24, 1998·Archives of Biochemistry and Biophysics·A I AlayashR E Cashon
Jul 2, 1998·Biochemical and Biophysical Research Communications·E Nagababu, J M Rifkind
Sep 28, 1998·Biochemistry·C BalagopalakrishnaJ M Rifkind
Jun 29, 1999·Nature Biotechnology·A I Alayash
Jul 17, 1999·The American Journal of Physiology·L L McLeod, A I Alayash
Jun 6, 2000·Advanced Drug Delivery Reviews·F D'Agnillo, A I Alayash
Dec 8, 2000·FEBS Letters·B HalliwellL H Long
Nov 24, 2001·Blood·F D'Agnillo, A I Alayash
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Susan H KwonWilson S Colucci
Sep 4, 2003·Journal of Agricultural and Food Chemistry·Charlotte U CarlsenLeif H Skibsted
Jan 20, 2004·Journal of Applied Physiology·Dan DrobinRobert M Winslow
Nov 23, 2006·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Jan SimoniDonald E Wesson
Mar 12, 2008·Antioxidants & Redox Signaling·Caroline Gaucher, Patrick Menu
Jan 10, 2009·Blood·Paul W BuehlerDominik J Schaer
Jan 31, 2009·Artificial Organs·Jan SimoniJohn F Moeller
Jan 31, 2009·Artificial Organs·Chris E Cooper
Oct 14, 2009·Chemical Research in Toxicology·María I González-SánchezEdelmira Valero
Oct 28, 2009·Anesthesiology·Toby A SilvermanUNKNOWN Planning Committee and the Speakers
Oct 4, 2011·Archives of Biochemistry and Biophysics·María I González-SánchezEdelmira Valero
Mar 19, 2014·Trends in Biotechnology·Abdu I Alayash
Apr 12, 2017·Antioxidants & Redox Signaling·Leif Bulow, Abdu I Alayash
May 20, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Mengdi WuKunping Yan
Jul 8, 2017·Advances in Experimental Medicine and Biology·Peter E Keipert
Nov 8, 2017·Shock·Abdu I Alayash
Jul 26, 2018·Artificial Organs·Qiuhui LiKunping Yan
Dec 16, 2019·Redox Biology·Flavia Dei ZottiJean-Luc Balligand