Fertility of unfrozen and frozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packaged in straws

Journal of Animal Science
P R LoomisB W Pickett

Abstract

The fertility of frozen-thawed semen was compared with that obtained using fresh semen extended in skim milk. Semen for freezing was obtained in June from four stallions of unknown fertility; two ejaculates were collected 1 to 2 h apart every 3 or 4 d. The gel-free fraction of the ejaculate was mixed 1:1 with a glucose-EDTA solution (disodium ethylenediaminetetraacetic acid) and centrifuged at 650 x g for 15 min. The spermatozoa were resuspended in an EDTA-lactose-egg yolk extender containing 5% glycerol. Semen was frozen in .5-ml French straws containing 250 x 10(6) progressively motile spermatozoa before freezing. Only for 31% of the 54 ejaculates frozen was post-thaw spermatozoal motility greater than or equal to 50% of the percentage of progressively motile spermatozoa observed during evaluation of the neat semen. Spermatozoa in second ejaculates apparently were more susceptible to the adverse effects of dilution and centrifugation than spermatozoa in first ejaculates. Only samples containing greater than 30% progressively motile spermatozoa after freezing and thawing (at 38 C) were used for insemination. In June and July, 101 mares were inseminated daily with semen from one of three stallions beginning on d 2 and continuin...Continue Reading

Citations

Jul 27, 2006·Molecular Reproduction and Development·Annick Bergeron, Puttaswamy Manjunath
Oct 13, 2006·Reproduction, Fertility, and Development·Rebecca E SpindlerDavid E Wildt
Jan 1, 1996·Equine Veterinary Journal·A V HeitlandC Hamilton
Jun 6, 2007·Australian Veterinary Journal·J R ClulowL H A Morris
Jul 13, 2005·Reproduction in Domestic Animals = Zuchthygiene·W R Allen
Nov 28, 2012·Animal Reproduction Science·Alberto ContriAugusto Carluccio

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