Fertilization and cleavage of mouse oocytes exposed to the conditions of human oocyte retrieval for in vitro fertilization

Fertility and Sterility
K V Jackson, A A Kiessling

Abstract

Ova from two strains of mice (a hybrid-inbred strain, B6D2F1, and a random-bred strain, CD1) were shocked by exposure to environmental conditions possibly encountered by human oocytes retrieved for in vitro fertilization (IVF). Shocked and control mouse ova were fertilized in vitro in either simple or complex media and zygote development to morulae and blastocyst stages compared with that of zygotes fertilized in vivo. Development of the hybrid-inbred zygotes following fertilization in the simple media of shocked and control ova was essentially the same as for ova fertilized in vivo (84 +/- 6.6, 89 +/- 1.6, 87 +/- 6.0% to the 2-cell stage and 89 +/- 5.2, 94 +/- 2.3, 99 +/- 1.0% of two cells to blastocysts, respectively); development in the complex media also was the same following fertilization of shocked and control ova (80 +/- 8.7, 90 +/- 2.7% to two cells and 33 +/- 3.5, 35 +/- 4.5% of two cells to blastocysts, respectively) but lower than that of in vivo zygotes (92 +/- 4.6 to two cells, 58 +/- 4.7 two cells to blastocysts). In contrast, the fertilization and development in the simple media of shocked and control random-bred ova was lower and more variable (90 +/- 5.8, 67 +/- 11.1% to two cells and 17 +/- 8.3, 42 +/- 13.6% ...Continue Reading

Citations

Apr 1, 1992·Journal of Assisted Reproduction and Genetics·F C GwazdauskasJ M Sreenan
Dec 1, 1991·Molecular Reproduction and Development·R MenkveldG D Hodgen
Jul 1, 1996·The Tohoku Journal of Experimental Medicine·P N Zarmakoupis-Zavos, P M Zavos
Feb 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·N HolstH I Nielsen
Oct 1, 1996·Journal of Assisted Reproduction and Genetics·M van den BerghY Englert
Jul 21, 1998·Molecular Reproduction and Development·B MaioneA A Kiessling
May 1, 1990·The Journal of Membrane Biology·M TonerD R Armant
Jan 1, 1994·Journal of Assisted Reproduction and Genetics·A D EsterhuizenJ A van Schouwenburg
Feb 6, 2008·Prostaglandins & Other Lipid Mediators·Maria SakellariouTheoxaris Patargias

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