PMID: 590661Oct 20, 1977

Fertilization and preimplantation development in vitro of mouse eggs obtained following stimulation with different doses of pregnant mare serum: a comparison of the responses in two strains

Differentiation; Research in Biological Diversity
L R Fraser


Unfertilized eggs were recovered from TO and (C57BL/10 X CBA)F1 females, induced to ovulate with 0.75--10.0 IU PMS and 5.0 IU HCG/mouse, and mixed with TO sperm in vitro. Among the groups of F1 eggs, there were no significant differences in either fertilization or development to the blastocyst stage in vitro. With TO eggs, however, there was significantly lower fertility in the 0.74 and 1.5 IU PMS groups than in the higher PMS dose groups. Conversely, preimplantation development was significantly better withe low doses. To determine whether polyploidy migh affect viability in vitro, digynic polyploidy was experimentally induced in both F1 and TO eggs with cytochalasin B. Development of polyploid F1 X TO embryos to the blastocyst stage did not differ significantly form that of untreated embryos. Development of polyploid TO X TO embryos obtained from eggs stimulated with 1.5 IU PMS was still higher than that for eggs stimulated with 7.5 IU PMS. This suggests that cytoplasmic inadequacies, rather than genome imbalance, are responsible for the reduced ability of the eggs stimulated with high doses of PMS to reach the blastocyst stage in vitro.


Dec 1, 1975·Journal of Reproduction and Fertility·H PetersM Faber
Jul 1, 1977·Journal of Reproduction and Fertility·I Maudlin, L R Fraser
Jul 1, 1976·The Journal of Experimental Zoology·L R Fraser, L M Drury
Nov 1, 1976·Journal of Reproduction and Fertility·A Niemierko, A Komar
Nov 18, 1976·Nature·N Takagi, M Sasaki
Jul 1, 1975·Journal of Reproduction and Fertility·A I Spindle, L S Goldstein
Dec 1, 1975·Biology of Reproduction·L R Fraser, L M Drury
Sep 1, 1974·Journal of Reproduction and Fertility·S FujimotoW R Dukelow
Apr 1, 1974·Journal of Reproduction and Fertility·N S Fechheimer, R A Beatty
Oct 1, 1971·Journal of Reproduction and Fertility·K ConradR Stambaugh
Mar 11, 1966·Science·A A Katzberg, A G Hendrickx
Oct 1, 1971·Journal of Reproduction and Fertility·J Allen, A McLaren
Feb 1, 1970·Biological Reviews of the Cambridge Philosophical Society·R A Beatty
Sep 1, 1957·The Journal of Endocrinology·R E FOWLER, R G EDWARDS


Dec 1, 1985·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M Condon-MahonyB Shirley
Feb 27, 2003·Biology of Reproduction·Catherine M H Combelles, David F Albertini
Sep 15, 2012·The Journal of Reproduction and Development·Safiriyu Idowu Ola, Qing-Yuan Sun
Jan 1, 1988·Annals of the New York Academy of Sciences·R H Foote, E W Carney
Mar 17, 2012·Biology of Reproduction·Monika A Ward

Related Concepts

Cytochalasin B
Polyspermic Fertilization
Pregnant Mare Serum Gonadotropins
Mice, Inbred CBA
Mice, Inbred C57BL
Polyploid Cell
Pregnancy, Animal
Prenidation Embryo Development, Animal

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