Fetal cell microchimerism in the maternal mouse spinal cord

Neuroscience Bulletin
Guohui ZhangJiming Kong

Abstract

Fetal cell microchimerism refers to the persistence of fetal cells in the maternal tissues following pregnancy. It has been detected in peripheral organs and the brain, but its existence in the spinal cord has not been reported. Our aim was to detect fetal cell microchimerism in the spinal cord of maternal mice. C57BL/6 female mice were crossed with GFP transgenic male mice and sacrificed after their first or third delivery. GFP-positive cells, which were presumably from fetuses whose fathers were GFP transgenic, were detected in the spinal cord by fluorescence microscopy and immunohistochemistry. PCR was also performed to detect GFP DNA, which must come from GFP hemizygous fetuses. We found GFP-positive cells and detectable GFP DNA in most of the maternal spinal cords. Twenty percent (1/5) of the mice that were only pregnant once had detectable fetal cells, while 80% (4/5) of those that were pregnant three times had detectable fetal cells. Some fetal cells, which not only emitted green fluorescence but also expressed NeuN, were detected in the spinal cords from maternal mice. These results indicate that fetal cells migrate into the spinal cord of a maternal mouse during and/or after the gestational period, and the fetal cells ...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L A HerzenbergG M Iverson
Sep 1, 1980·Journal of Neuropathology and Experimental Neurology·H Barden
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BianchiM A DeMaria
Jan 22, 1996·American Journal of Medical Genetics·B A van DijkA J de Man
May 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A SchnellM W Wessendorf
Aug 14, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Carol M ArtlettJ Bruce Smith
Oct 27, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Anne M StevensJ Lee Nelson
Nov 16, 2004·Biochemical and Biophysical Research Communications·Yu WangMasatsugu Hori
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Marije KoopmansIngeborg M Bajema
Jun 14, 2005·Journal of Reproductive Immunology·Kiarash KhosrotehraniDiana W Bianchi
May 29, 2007·International Review of Neurobiology·Meghan KennerDebra G Elliott
Sep 28, 2007·The European Journal of Neuroscience·Eva HedlundOle Isacson
Oct 3, 2007·Cancer Research·Vijayakrishna K Gadi, J Lee Nelson
May 30, 2009·The Tohoku Journal of Experimental Medicine·Keiichi MatsubaraMasaharu Ito

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Citations

Dec 7, 2017·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Abdol-Mohammad KajbafzadehNastaran Sabetkish
Sep 30, 2020·Annals of the New York Academy of Sciences·Sangeetha Vadakke-Madathil, Hina W Chaudhry

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