Fetal cells in transcervical samples at an early stage of gestation

Journal of Human Genetics
M Adinolfi, J Sherlock

Abstract

Several investigations are in progress with the aim of performing prenatal diagnosis of inherited disorders by noninvasive or minimally invasive techniques. The most important approaches are based on the detection of fetal nucleated cells in maternal blood, the analysis of fetal DNA present in maternal plasma, and the identification and isolation of fetal trophoblastic cellular elements shed into the uterine cavity and the endocervical canal. In this review, we discuss the methods that have been employed for the collection of the transcervical samples at an early stage of gestation and the techniques used for the identification of fetal cells. We also report the results of using endocervical cells for the detection of fetal chromosomal disorders by fluorescent in-situ hybridization and for performing prenatal diagnosis of fetal Rh(D) phenotypes. Recent investigations have also shown that--after the isolation of trophoblastic cells from maternal contaminants by micromanipulation--transcervical samples can be employed for the prenatal diagnosis of single gene defects, such as those causing thalassemia and sickle cell anemia. Although the present results are promising, further investigations are required to demonstrate the feasibi...Continue Reading

Citations

Aug 7, 2012·Veterinary Research Communications·Mohammad Reza DivarMojtaba Kafi
Oct 10, 2003·The New England Journal of Medicine·Michael T Mennuti, Deborah A Driscoll
Feb 28, 2003·Pharmacogenomics·Rossa W K Chiu, Y M Dennis Lo
May 18, 2010·Early Human Development·Stavros SifakisAliza Amiel
Dec 14, 2005·The Journal of Obstetrics and Gynaecology Research·Chun-Neng FangChang-Chun Hsiao
Jan 13, 2006·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Debbie MantzarisGab Kovacs
Aug 9, 2001·Prenatal Diagnosis
Jul 18, 2003·Prenatal Diagnosis·Xiao Xi ZhaoTakeshi Sato
Dec 11, 2002·AACN Clinical Issues·Maryann Bozzette

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