PMID: 3756190Sep 12, 1986Paper

Fetal lung surfactant lipid synthesis from glycogen during organ culture

Biochimica Et Biophysica Acta
P M Farrell, J Bourbon

Abstract

The role of fetal lung glycogen as a precursor for lipids during late gestational development was explored by a combination of in vivo labeling with [U-14C]glucose, administered directly to rat fetuses at 18.5 days, and in vitro assessment using an organ explant culture system. Our major objectives were to demonstrate that radioactivity was transferred specifically and preferentially to surfactant lipids, as glycogenolysis occurred, and to determine the molecular distribution of 14C labeling in newly synthesized phosphatidylcholine (PC). Surfactant and residual (non-surfactant) lipids were separated by sucrose density gradient centrifugation, and other subcellular fractions such as microsomes were isolated by subsequent centrifugations. After 72 h of culture, there was a 5.7-fold increase in the concentration of PC in the surfactant fraction, which contributed 8.8% of total PC at the beginning and 29.6% (P less than 0.001) at the end of the 72 h period. The labeling of PC in the surfactant fraction increased markedly during culture, but there was no significant change in the residual fraction or microsomal PC. Hydrolysis of surfactant PC indicated that the radioactivity was predominantly located in the fatty acyl portion of the...Continue Reading

References

Sep 28, 1978·Biochimica Et Biophysica Acta·W M ManiscalcoJ B Warshaw
May 17, 1976·Biochemical and Biophysical Research Communications·J D FunkhouserR D Peterson
Jan 28, 1985·Biochimica Et Biophysica Acta·G RijksenL M van Golde
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Aug 18, 1982·Biochimica Et Biophysica Acta·J R BourbonP M Farrell
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Anne-Marie CanakisHugh O'Brodovich
Aug 5, 2003·American Journal of Respiratory Cell and Molecular Biology·Feijie ZhangRobert J Mason
Oct 6, 2005·Brain Research·Stephan VerleysdonkBernd Hamprecht
Mar 1, 1989·Experimental Lung Research·N GuettariC Tordet
Jan 1, 1988·Experimental Lung Research·M J EngleM Dooley
Jul 1, 1991·Experimental Lung Research·J R BourbonJ M Johnston
May 29, 1987·Biochemical and Biophysical Research Communications·M J EngleD J Brown
Nov 9, 2004·Pediatric Pulmonology·Chung-Ming ChenTsu-Fuh Yeh
Aug 1, 1988·The Anatomical Record·A A Ten Have-OpbroekC J Otto-Verberne
Jan 27, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·William RaoulJacques R Bourbon
Jan 13, 1987·Biochimica Et Biophysica Acta·J R BourbonM Rieutort
Apr 8, 1999·Progress in Lipid Research·J J Batenburg, H P Haagsman
Jun 9, 1988·Biochimica Et Biophysica Acta·M Post, L M van Golde

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