Fetal Membrane Organ-On-Chip: An Innovative Approach to Study Cellular Interactions

Reproductive Sciences
Lauren RichardsonRamkumar Menon

Abstract

Fetal membranes, a vital component that helps maintain pregnancy and contribute to parturition signaling, are often studied in segments due to its structural complexity. Transwells are traditionally used to study cell interactions; however, their usefulness is limited. To overcome these difficulties, a fetal membrane-organ-on-chip (FM-OO-C) was created to study interactive properties of amnion epithelial cells (AECs) and decidual cells compared to transwell systems. Primary AECs and decidual cells from term, nonlaboring fetal membranes were cultured in a 2-chamber (AEC/decidual cell) FM-OO-C device and sandwiched between a semipermeable membrane. Cells were treated with cigarette smoke extract (CSE) or dioxin, and membrane permeability and cellular senescence were measured after 48 hours. The same experiments were conducted in transwells for comparisons. Compared to transwell cultures, FM-OO-C model produced better membrane permeability readings regardless of the side of treatment or time point. Membrane permeabilization was higher in AECs directly treated with CSE (1.6 fold) compared to similar treatment on the decidual side (1.2 fold). In FM-OO-C, treatments forced changes between cellular layers. This was evident when CSE an...Continue Reading

References

May 18, 2006·Reproductive Biology and Endocrinology : RB&E·Yumiko AbeKaoru Miyamoto
Jan 5, 2014·PloS One·Ramkumar MenonRobert N Taylor
Apr 2, 2015·Annals of the American Thoracic Society·Dongeun Dan Huh
May 31, 2015·American Journal of Obstetrics and Gynecology·Faranak BehniaRamkumar Menon
Jun 13, 2015·Biomedical Materials·Yu Shrike ZhangAli Khademhosseini
May 4, 2016·Tissue Barriers·Marinke W van der HelmLoes I Segerink
Jul 2, 2016·Human Reproduction Update·Ramkumar MenonRobert N Taylor
Jan 4, 2017·American Journal of Reproductive Immunology : AJRI·Anjali P AndersDavid M Aronoff
Jun 16, 2017·Physiology·Kayla DuvalZi Chen
Aug 30, 2017·Current Pharmaceutical Design·Juan S GneccoDavid M Aronoff
Dec 2, 2017·American Journal of Reproductive Immunology : AJRI·Christopher Luke DixonRamkumar Menon
Jul 10, 2018·The American Journal of Pathology·Lauren Richardson, Ramkumar Menon
Aug 8, 2018·American Journal of Reproductive Immunology : AJRI·Lisa M RogersDavid M Aronoff

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Citations

May 1, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lauren RichardsonRamkumar Menon
Apr 30, 2020·Tissue Engineering. Part a·Hannes CampoJ Julie Kim
Jun 18, 2020·Frontiers in Physiology·Sean M HarrisRita Loch-Caruso
Oct 29, 2020·Lab on a Chip·Lauren S RichardsonRamkumar Menon

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