Fetal pulmonary immunoreactive adrenocorticotropin: molecular weight and cellular localization

Regulatory Peptides
Charles E WoodM I Castro

Abstract

The hypothalamus-pituitary-adrenal axis of the sheep fetus plays a critical role in fetal development, responsiveness to stress, and initiation of parturition. We have recently reported that the fetal lung contains and secretes significant amounts of immunoreactive adrenocorticotropin (iACTH). The present study was designed to identify the molecular weight profile and the cellular location of iACTH in this tissue. iACTH extracted from fetal lung was immunoprecipitated, electrophoresed, and immunoblotted. Pulmonary iACTH was found in several molecular forms. The largest peptides appeared as doublets, and had molecular weights similar to POMC (32, 33 kD). Smaller peptides appeared in molecular weights (17, 24, and 27 kD) which were not consistent with the post-translational processing of POMC in fetal pituitary, but which were consistent with known processing of POMC by chromaffin granule aspartyl protease. None of the molecular forms of iACTH were glycosylated. Immunohistochemistry revealed that the iACTH was contained within bronchial epithelium and within groups of cells within the parenchyma of the lung. Both of these types of cells are consistent with pulmonary neuroendocrine cells. The distribution of neuroendocrine cells a...Continue Reading

References

Apr 1, 1991·Domestic Animal Endocrinology·M E BellM Keller-Wood
May 1, 1993·The Anatomical Record·D Adriaensen, D W Scheuermann

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Citations

Mar 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Charles E WoodMaureen Keller-Wood
Jan 29, 2011·Reproductive Sciences·Jared WinikorCharles E Wood
Nov 6, 2010·Reproductive Biology and Endocrinology : RB&E·Marc SimardYves Tremblay
Feb 8, 2005·Journal of the Society for Gynecologic Investigation·Charles E Wood
Oct 18, 2003·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·S RanganathanR Jaffe
Dec 25, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dean A MyersCharles A Ducsay
Mar 17, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Aly G SaadJerzy Stanek

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