PMID: 7517900Apr 1, 1994Paper

Fetal rat glandular stomach epithelial cells differentiate into surface mucous cells which express cathepsin E in the absence of mesenchymal cells in primary culture

Differentiation; Research in Biological Diversity
H FukamachiK Miki

Abstract

It is well established that the differentiation of glandular stomach epithelial cells is affected by many factors including epithelial-mesenchymal interactions. To clarify the control mechanism of their differentiation, we developed a primary culture system for fetal rat glandular stomach epithelial cells, and examined their differentiation in the absence of mesenchyme. Pure glandular stomach epithelial tissues obtained from 16.5-day fetal rats proliferated rapidly, increasing their number about 20 times in the first 7 days. The epithelial nature of the cells was confirmed by the presence of cytokeratin in the cells. Glandular stomach epithelial cells formed simple cuboidal/squamous epithelia with many mucous granules in their cytoplasm, and exhibited epithelial polarity with microvilli on the luminal surface, basal lamina-like material on the basal surface, and junctional complexes in the apical region. Biochemical analysis showed that the cells expressed acid protease activity in culture. Previous studies showed that glandular stomach epithelial cells specifically expressed two types of acid proteases: pepsinogens in chief and mucous neck cells, and cathepsin E in surface mucous cells. Immunohistochemical studies using specif...Continue Reading

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Citations

Oct 23, 1998·Development, Growth & Differentiation·H Tabata, S Yasugi
Sep 11, 2004·Biochemical and Biophysical Research Communications·Hiroshi FukamachiYoshiaki Ito
Dec 10, 1999·Experimental Cell Research·J R BasqueD Ménard
Apr 7, 2010·The American Journal of Pathology·Lina UddTomi P Mäkelä
Nov 5, 1999·In Vitro Cellular & Developmental Biology. Animal·H PuM Sakaguchi
Apr 24, 2008·Development, Growth & Differentiation·Sadao Yasugi, Takeo Mizuno
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Toshio NakajimaToshiyuki Takeuchi
Nov 17, 2005·Development, Growth & Differentiation·Hiroshi FukamachiMasao Ichinose
Jan 23, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Susumu MatsushitaSadao Yasugi
Aug 17, 1999·Differentiation; Research in Biological Diversity·T Koike, S Yasugi

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