Fetal rat metabonome alteration by prenatal caffeine ingestion probably due to the increased circulatory glucocorticoid level and altered peripheral glucose and lipid metabolic pathways

Toxicology and Applied Pharmacology
Yansong LiuHui Wang

Abstract

The aims of this study were to clarify the metabonome alteration in fetal rats after prenatal caffeine ingestion and to explore the underlying mechanism pertaining to the increased fetal circulatory glucocorticoid (GC). Pregnant Wistar rats were daily intragastrically administered with different doses of caffeine (0, 20, 60 and 180 mg/kg) from gestational days (GD) 11 to 20. Metabonome of fetal plasma and amniotic fluid on GD20 were analyzed by ¹H nuclear magnetic resonance-based metabonomics. Gene and protein expressions involved in the GC metabolism, glucose and lipid metabolic pathways in fetal liver and gastrocnemius were measured by real-time RT-PCR and immunohistochemistry. Fetal plasma metabonome were significantly altered by caffeine, which presents as the elevated α- and β-glucose, reduced multiple lipid contents, varied apolipoprotein contents and increased levels of a number of amino acids. The metabonome of amniotic fluids showed a similar change as that in fetal plasma. Furthermore, the expressions of 11β-hydroxysteroid dehydrogenase 2 (11β-HSD-2) were decreased, while the level of blood GC and the expressions of 11β-HSD-1 and glucocorticoid receptor (GR) were increased in fetal liver and gastrocnemius. Meanwhile, ...Continue Reading

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Citations

Jan 8, 2013·European Journal of Nutrition·Diana F Serapiao-MoraesTatiane S Faria
Sep 15, 2015·Toxicology and Applied Pharmacology·Yi-Meng WuHui Wang
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Oct 13, 2016·Basic & Clinical Pharmacology & Toxicology·Yang Quan-JunGuo Cheng
May 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dan XuHui Wang
Jan 20, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Xuan XiaHui Wang

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