Fetal rat pancreas in organ culture: effects of serum on the development of the endocrine cells

Differentiation; Research in Biological Diversity
R C McEvoy

Abstract

Eighteen-day-old rat fetal pancreas was grown in organ culture for four days on medium consisting of tissue culture Medium 199 and varying concentrations of chicken serum. The glucagon and somatostatin concentration of the explants was decreased when the serum concentration of the medium was reduced from 50 to 10%. There was a further reduction in these hormones when the explants were cultured on Medium 199 alone. Explant insulin content was reduced only when serum was omitted from the medium. A "serum factor" tripeptide was not able to substitute for this serum requirement. Heat-inactivation of the serum resulted in a significant increase in medium insulin content and an increase in both the insulin and glucagon contents of the explants. This increase in hormone content was directly correlated with increases in the beta and alpha cell volumes of the explants. There was no change in the somatostatin content or delta cell volume of the explants grown on heat-inactivated medium. It is suggested that the serum is an important component of the culture media and is apparently required in high concentration for the development of the islet cells in vitro. The islet cell types differ in their requirement for serum. The alpha and delta...Continue Reading

References

Jul 1, 1977·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R C McEvoyR P Elde
Mar 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·A ArimuraA V Schally
Jan 1, 1971·Analytical Biochemistry·R T Hinegardner
Jan 1, 1960·Scandinavian Journal of Clinical and Laboratory Investigation·E RAABO, T C TERKILDSEN

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Citations

Apr 1, 1985·General and Comparative Endocrinology·J Hulsebus, E S Farrar
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·K C Kerns, E S Farrar
Aug 10, 2011·Nature Reviews. Rheumatology·Lesley Davila, Prabha Ranganathan
Dec 29, 2013·European Journal of Clinical Pharmacology·Sunitha KodidelaBiswajit Dubashi
Feb 2, 2019·Current Drug Metabolism·Guillermo Gervasini, Sonia Mota-Zamorano

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