PMID: 8942854Dec 1, 1996Paper

Fetal RhD typing by polymerase chain reaction in pregnancies complicated by rhesus alloimmunization

Obstetrics and Gynecology
I B Van den Veyver, K J Moise

Abstract

To review the specificity and sensitivity of diagnostic techniques using the polymerase chain reaction (PCR) on amniotic fluid (AF) samples for the determination of fetal RhD status. A MEDLINE computerized search was conducted for January 1991 through March 1996 using the key terms "polymerase chain reaction," "rhesus," and "RhD typing." All articles describing the use of PCR in AF for RhD typing were reviewed. Only cases in which the results of PCR testing were confirmed by fetal or neonatal serology were included in the final analysis. The results of PCR typing were compared with serology to determine the sensitivity, specificity, and positive and negative predictive values of DNA-based techniques. A total of 500 cases were reviewed, in which four different sets of oligonucleotide primers were used. The sensitivity and specificity of PCR typing were 98.7% and 100%, respectively, and the positive and negative predictive values were 100% and 96.9%, respectively. In five cases, an RhD-positive fetus was incorrectly diagnosed: Two fetuses died, one neonate needed exchange transfusions, and another neonate needed phototherapy in conjunction with a simple transfusion. The remaining infant was lost to follow-up. A theoretical model ...Continue Reading

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Citations

Sep 11, 2002·Obstetrics and Gynecology·Kenneth J Moise
Jan 9, 1999·Transfusion Medicine·W A FlegelC Gassner
Apr 29, 1998·Current Opinion in Obstetrics & Gynecology·I B Van den Veyver, B B Roa
Nov 29, 2001·Journal of Obstetric, Gynecologic, and Neonatal Nursing : JOGNN·J L Neal
Nov 29, 2011·Best Practice & Research. Clinical Obstetrics & Gynaecology·Michael EgborAmarnath Bhide
Jul 26, 2005·Seminars in Hematology·Kenneth J Moise
Nov 3, 2004·Clinics in Perinatology·Ursula F Harkness, Joseph A Spinnato
Mar 9, 2006·American Journal of Obstetrics and Gynecology·Henry Roque
Apr 1, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·V L Bills, P W Soothill
Aug 7, 2017·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·N AbbasiG Ryan
Jul 2, 2008·Obstetrics and Gynecology·Kenneth J Moise
Aug 22, 2018·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·S PlešinacI Babović
May 3, 2000·Seminars in Hematology·C H HuangJ G Cheng

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