FETAX interlaboratory validation study: phase III--Part 1 testing

Journal of Applied Toxicology : JAT
J A BantleS D Turley

Abstract

The Frog Embryo Teratogenesis Assay-Xenopus (FETAX) is a 96-h whole embryo developmental toxicity screening assay that can be used in ecotoxicology and in detecting mammalian developmental toxicants when an in vitro metabolic activation system is employed. A standardized American Society for Testing and Materials (ASTM) guide for the conduct of FETAX has been published, along with a companion atlas that aids in embryo staging and identifying malformations. As part of the ASTM process, a three-phase interlaboratory validation study was undertaken to evaluate the repeatability and reliability of FETAX. Seven different participants collaborated in the study. In Phase I, FETAX proved to be more repeatable and reliable than many bioassays. However, some excessive variation was observed in a few laboratories. An initial lack of assay experience by some technicians caused variation. Phase II showed far less intra- and interlaboratory variability than Phase I. Non-teratogens showed the most consistent results, while more variability was observed for the two teratogens tested. Interlaboratory coefficient of variation values for all endpoints ranged from 7.3 to 54.7. Phase III--Part 1, using coded samples and test concentration ranges se...Continue Reading

Citations

Sep 26, 2006·Archives of Environmental Contamination and Toxicology·Douglas J FortChristian Schlekat
Sep 8, 2010·Ecotoxicology·M Gabriela AgostiniAlicia E Ronco
Nov 1, 2000·Chemosphere·M PratiG Bernardini
Jul 16, 2005·Journal of Toxicology and Environmental Health. Part a·Shannon K LeeD N Rao Veeramachaneni
Jun 17, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeremy A LavineWade H Powell
Jan 5, 2021·Toxicology Reports·Mark PerfettoJennifer E G Gallagher
Oct 18, 2002·Toxicology and Applied Pharmacology·Nazzareno Ballatori, Alice R Villalobos
Nov 20, 2004·Journal of Applied Toxicology : JAT·Douglas J FortClinton D Spaulding

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