Jan 1, 1977

Feulgen type staining of mammalian tissues with Dahlia-SO2

Folia Histochemica Et Cytochemica
M K Dutt

Abstract

The use of the basic dye, Dahlia, which belongs to triphenylmethane group but without a primary amino group in its molecule has been described as useful in the staining of aldehyde groups of acid hydrolysed DNA in tissue sections following the conventional Feulgen procedure. Dahlia-SO2 prepared with sodium hydrosulphite is highly suitable when used at pH 4-0 to 5-0. The absorption characteristics of the stained nuclei indicate on the peak of maximum absorption at 560 nm, whereas, that of the aqueous dye solution is at 590 nm.

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Mentioned in this Paper

Triphenylmethane
Kidney
Cell Nucleus
Aldehydes
Staining and Labeling
Dahlia
Organic Pigments
Staining Method
Liver
Trityl Compounds

About this Paper

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