FGF receptor-mediated gene delivery using ligands coupled to PEI-β-CyD.

Journal of Biomedicine & Biotechnology
Yiping HuPeng Zhang

Abstract

A novel vector with high gene delivery efficiency and special cell-targeting ability was developed using a good strategy that utilized low-molecular-weight polyethylenimine (PEI; molecular weight: 600 KDa [PEI600]) crosslinked to β-cyclodextrin (β-CyD) via a facile synthetic route. Fibroblast growth factor receptors (FGFRs) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy. In this paper, CY11 peptides, which have been proven to combine especially with FGFRs on cell membranes were coupled to PEI-β-CyD using N-succinimidyl-3-(2-pyridyldithio) propionate as a linker. The ratios of PEI600, β-CyD, and peptide were calculated based on proton integral values obtained from the (1)H-NMR spectra of the resulting products. Electron microscope observations showed that CY11-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer. Experiments on gene delivery efficiency in vitro showed that CY11-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the COS-7 and HepG2 cells, which positively expressed FGFR, whereas no such effect was observed in the PC-3...Continue Reading

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Citations

Apr 23, 2013·Advanced Drug Delivery Reviews·Hidetoshi ArimaTaishi Higashi
Jul 19, 2013·BioMed Research International·Lara ProsenGregor Sersa
Oct 10, 2013·Biomaterials·Qiwen WangPaul K Chu
Oct 10, 2013·Biomaterials·Wing-Fu Lai
Sep 11, 2013·Developmental Biology·Stephen J GauntYu-Lee Paul

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Methods Mentioned

BETA
transfection
NMR
Electrophoresis
FCS
protein assay
Assay
phage display

Software Mentioned

SPSS12

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