FGF2-stimulated release of endogenous FGF1 is associated with reduced apoptosis in retinal pigmented epithelial cells

Experimental Cell Research
X GuillonneauF Mascarelli

Abstract

Both inhibition of endogenous fibroblast growth factor (FGF) synthesis on nondividing lens epithelial cells and inhibition of secreted FGF1 in confluent quiescent retinal pigmented epithelial (RPE) cells induce rapid cell apoptosis (Renaud et al., 1996, J. Biol. Chem., 271, 2801-2811). In addition several studies demonstrate that exogenous FGF2 can promote retinal cell survival in vitro and in vivo. To determine the possible relationship between exogenous FGF2, endogenous FGF1, and cell survival, we examined the protective effect of a single dose of exogenous FGF2 on long-term culture of quiescent RPE cells after serum withdrawal. After 4 days of culture, a dramatic and sustained upregulation of FGF1 protein expression occurs specifically in response to exogenous FGF2. After addition of FGF2 (20 ng/ml), RPE cells express fourfold more FGF1 after Day 7 than after Day 1 of culture. This phenomenon is FGF2 dose-dependent. In contrast, neither serum nor FGF2 have an effect on total endogenous FGF2 expression. In addition, in response to exogenous FGF2, FGF1 is secreted in significant amounts into the extracellular medium at a rate comparable to FGF1 accumulation within the cell. Furthermore, in the absence of serum, significant inc...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A JacksonT Maciag
Jan 1, 1992·Progress in Growth Factor Research·J PartanenK Alitalo
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A TanakaK Matsumoto
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M M LaVailR H Steinberg
Jul 1, 1989·The Journal of Cell Biology·D B Rifkin, D Moscatelli
Jan 1, 1989·Annual Review of Biochemistry·W H Burgess, T Maciag
Feb 1, 1989·Journal of Cellular Biochemistry·D CaruelleD Barritault
Mar 30, 1987·Biochemical and Biophysical Research Communications·L SchweigererD Gospodarowicz
Mar 1, 1994·Journal of Cellular Physiology·F RenaudM Laurent
Jul 1, 1995·Current Eye Research·J PerryP Gouras
Mar 1, 1993·Journal of Cellular Physiology·F MalecazeD Hicks
Mar 1, 1995·Journal of Cellular Physiology·R Z FlorkiewiczE Florkiewicz
Feb 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M K CollinsA López-Rivas
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C Portera-CailliauR Adler
Feb 1, 1993·Endocrinology·A Baird
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F R SteeleJ Tombran-Tink
May 24, 1996·The Journal of Biological Chemistry·J C Fox, J R Shanley

❮ Previous
Next ❯

Citations

Feb 24, 2004·Annual Review of Physiology·John M Shannon, Brian A Hyatt
Sep 23, 1997·Biochemical and Biophysical Research Communications·T LuckcuckJ H Walker
Oct 12, 2000·Radiation Research·P Y ChangE A Blakely
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R RosenthalO Strauss
Nov 9, 2000·Progress in Retinal and Eye Research·G M HoltkampA F de Vos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis