FgRIC8 is involved in regulating vegetative growth, conidiation, deoxynivalenol production and virulence in Fusarium graminearum

Fungal Genetics and Biology : FG & B
Jinjin WuZhengyi Wang

Abstract

Proteins of the resistance to inhibitors of cholinesterase 8 (Ric8) group act as guanine nucleotide exchange factors (GEFs) and play important roles in regulating G-protein signaling in animals. In filamentous fungi, putative Ric8 orthologs have so far been identified in Magnaporthe oryzae, Neurospora crassa, Aspergillus nidulans and Aspergillus fumigatus. Here, we report the functional investigation of a potential RIC8 ortholog (FgRIC8) in the wheat head blight pathogen Fusarium graminearum. Targeted gene deletion mutants of FgRIC8 exhibited a significant reduction in vegetative growth, conidiation, pigment production as well as deoxynivalenol (DON) biosynthesis. Pathogenicity assays using a point-inoculated spikelet approach showed that the mutants were severely impaired in virulence on flowering wheat heads. Quantitative RT-PCR analysis revealed that genes encoding F. graminearum Gα (FgGpa1 and FgGpa3), Gβ (FgGpb1) and Gγ (FgGpg1) subunits were significantly down-regulated in Fgric8 mutants. Moreover, we showed that FgRic8 physically interacts with both FgGpa1 and FgGpa3, but not FgGpa2, in yeast two-hybrid assays. The intracellular cAMP levels in Fgric8 mutants were significantly decreased compared to the isogenic wild-type...Continue Reading

References

Dec 1, 1994·Genes & Development·S GoldJ Kronstad
Jul 1, 1995·Molecular Plant-microbe Interactions : MPMI·R H ProctorS P McCormick
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·K G MillerJ B Rand
Dec 9, 1997·Molecular Plant-microbe Interactions : MPMI·S Liu, R A Dean
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 1, 2002·Science·Susana R NevesRavi Iyengar
Jan 2, 2003·The Journal of Biological Chemistry·Gregory G TallAlfred G Gilman
Jul 31, 2004·Annual Review of Phytopathology·Guihua Bai, Gregory Shaner
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Oct 18, 2005·Nature Cell Biology·Bernhard HampoelzJuergen A Knoblich
Oct 26, 2005·Current Biology : CB·Thomas M Wilkie, Lisa Kinch
Apr 25, 2007·Fungal Genetics and Biology : FG & B·David E StarkeyKerry O'Donnell
May 18, 2007·Annual Review of Microbiology·Liande LiKatherine A Borkovich
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Julia SchumacherBettina Tudzynski
Nov 1, 2004·Molecular Plant Pathology·Rubella S Goswami, H Corby Kistler
Nov 1, 2002·Molecular Plant Pathology·Annett KlimpelBettina Tudzynski
Sep 1, 2003·Molecular Plant Pathology·Martin UrbanKim Hammond-Kosack
May 18, 2012·Molecular Plant-microbe Interactions : MPMI·Nguyen Van ThuatJörg Bormann
May 29, 2012·Fungal Genetics and Biology : FG & B·Ae Ran ParkYin-Won Lee
Jun 21, 2012·Fungal Genetics and Biology : FG & B·Jinhua JiangZhonghua Ma
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rama KatariaArjan Kortholt
Jan 24, 2014·Molecular Plant-microbe Interactions : MPMI·Shuai HuJin-Rong Xu

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