Fhl-1, a new key protein in pulmonary hypertension

Circulation
Grazyna KwapiszewskaNorbert Weissmann

Abstract

Pulmonary hypertension (PH) is a severe disease with a poor prognosis. Different forms of PH are characterized by pronounced vascular remodeling, resulting in increased vascular resistance and subsequent right heart failure. The molecular pathways triggering the remodeling process are poorly understood. We hypothesized that underlying key factors can be identified at the onset of the disease. Thus, we screened for alterations to protein expression in lung tissue at the onset of PH in a mouse model of hypoxia-induced PH. Using 2-dimensional polyacrylamide gel electrophoresis in combination with matrix-assisted laser desorption/ionization time-of-flight analysis, we identified 36 proteins that exhibited significantly altered expression after short-term hypoxic exposure. Among these, Fhl-1, which is known to be involved in muscle development, was one of the most prominently upregulated proteins. Further analysis by immunohistochemistry, Western blot, and laser-assisted microdissection followed by quantitative polymerase chain reaction confirmed the upregulation of Fhl-1, particularly in the pulmonary vasculature. Comparable upregulation was confirmed (1) after full establishment of hypoxia-induced PH, (2) in 2 rat models of PH (mo...Continue Reading

References

Sep 28, 1990·Biochemical and Biophysical Research Communications·M MugurumaT Fukazawa
Apr 19, 1984·Nature·K Burridge, P Mangeat
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·R FeuersteinS A Liebhaber
Jun 13, 2000·Advances in Experimental Medicine and Biology·G L SemenzaA Yu
Nov 7, 2000·Current Opinion in Cell Biology·S V Ekholm, S I Reed
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Taraseviciene-StewartR M Tuder
Dec 26, 2001·Pharmacology & Therapeutics·T K Jeffery, J C Wanstall
Jan 12, 2002·The American Journal of Pathology·Ludger FinkRainer Maria Bohle
Oct 25, 2002·American Journal of Physiology. Cell Physiology·Paul A RobinsonChristina A Mitchell
Feb 7, 2003·Journal of Cell Science·Amanda S CouttsDonald M Black
Jun 15, 2004·Journal of the American College of Cardiology·Marc HumbertMarlene Rabinovitch
Jun 29, 2005·The Journal of Cell Biology·Andrew SmithNancy Hogg
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·B I RatnikovM H Ginsberg
Aug 23, 2005·Proceedings of the American Thoracic Society·Gregg L Semenza
Aug 27, 2005·Seminars in Respiratory and Critical Care Medicine·C Gregory Elliott
Oct 4, 2005·The Journal of Clinical Investigation·Ralph Theo SchermulyFriedrich Grimminger
Nov 3, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bastian EulJörg Hänze
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Weiling XuSerpil C Erzurum

❮ Previous
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Citations

Jan 6, 2012·The Journal of Biological Chemistry·Maimon E HubbiGregg L Semenza
Mar 7, 2009·The Journal of Biological Chemistry·Nicole A NeumanAkiko Hata
May 17, 2013·Tissue Engineering. Part a·Karolina Janeczek PortalskaJan de Boer
Apr 24, 2012·Human Molecular Genetics·Felix W FriedrichLucie Carrier
Feb 9, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amy L FirthJason X-J Yuan
May 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Danielle L BurkeKurt R Stenmark
Nov 17, 2011·American Journal of Respiratory and Critical Care Medicine·Norbert Weissmann
Apr 2, 2011·American Journal of Respiratory Cell and Molecular Biology·Lunyin Yu, Charles A Hales
Feb 12, 2014·International Journal of Medical Sciences·Li-Li WangZheng-Wei Yuan
Mar 29, 2014·Molecular Medicine·Patrick Crosswhite, Zhongjie Sun
Jun 22, 2011·Nature Reviews. Cardiology·Ralph T SchermulyFriedrich Grimminger
Dec 1, 2010·Respiration; International Review of Thoracic Diseases·Seiichiro Sakao, Koichiro Tatsumi
Oct 29, 2011·Pulmonary Circulation·Michael E YeagerKurt R Stenmark
Sep 26, 2015·Pulmonary Pharmacology & Therapeutics·Rita Nogueira-FerreiraTiago Henriques-Coelho
Sep 14, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Wei Wu, Naftali Kaminski
Sep 30, 2010·Journal of Cellular and Molecular Medicine·Thiruchelvi ShathasivamAnthony O Gramolini
May 2, 2014·British Journal of Pharmacology·S CrnkovicG Kwapiszewska
Nov 20, 2014·Proteomics. Clinical Applications·Kelley L Colvin, Michael E Yeager
Oct 29, 2014·Proteomics. Clinical Applications·Melanie K NiesAllen D Everett
Oct 13, 2012·The American Journal of Pathology·Grazyna KwapiszewskaLudger Fink
Sep 11, 2012·The American Journal of Pathology·Christine VeithGrażyna Kwapiszewska
Jun 5, 2010·American Journal of Respiratory and Critical Care Medicine·Matthias HeckerRory E Morty
Feb 13, 2018·The Journal of Cell Biology·Xiang WangHongquan Zhang
Nov 9, 2017·Cellular and Molecular Life Sciences : CMLS·Lukasz WujakMalgorzata Wygrecka
May 1, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Junqiu YueGuoping Wang
Nov 3, 2010·Circulation·Vahitha B Abdul-SalamMartin R Wilkins
Aug 28, 2012·Circulation. Cardiovascular Genetics·Josepha S BinderChristian Windpassinger
Dec 19, 2018·Circulation. Genomic and Precision Medicine·Caroline J CoatsPerry M Elliott
Aug 6, 2016·The European Respiratory Journal·Elisabetta GamenSoni Savai Pullamsetti
Feb 9, 2010·Expert Review of Cardiovascular Therapy·Khaled AbdelhadyMalek G Massad
May 4, 2013·The Journal of Biological Chemistry·Qiulin TanFrank S Lee
Oct 27, 2017·Experimental and Therapeutic Medicine·Yanna DuXindong Xue
Sep 27, 2018·Nature Communications·Lauren E ByrnesJulie B Sneddon
Jan 26, 2020·Basic Research in Cardiology·Christine VeithAkylbek Sydykov
Jun 21, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tingting ShenDaling Zhu

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