FHL2 mediates tooth development and human dental pulp cell differentiation into odontoblasts, partially by interacting with Runx2

Journal of Molecular Histology
Jianxin DuXiaoying Wang

Abstract

The differentiation of mesenchymal cells in tooth germ and dental pulp cells into odontoblasts is crucial for dentin formation, and the transcription factor runt-related transcription factor (Runx2) is necessary for odontoblast differentiation. Our previous study demonstrated that four and a half LIM domains 2 (FHL2) may play an important role in tooth development and human dental pulp cell differentiation. This study aimed to determine whether FHL2 mediated the mesenchymal cells in tooth development and human dental pulp cell differentiation into odontoblasts by interacting with Runx2. The expression patterns of FHL2 and Runx2 were examined at the early stages of mouse molar development using double immunofluorescence staining. Western blot analysis and co-immunoprecipitation (Co-IP) were conducted for the preliminary study of the relationship between FHL2 and Runx2 in human dental pulp cell differentiation into odontoblasts. Results of double immunofluorescence staining showed that FHL2 and Runx2 exhibited similar expression patterns at the early stages of tooth development. Western blot analysis indicated that the expression patterns of FHL2 and Runx2 were synchronized on day 7 of induction, whereas those on day 14 differed....Continue Reading

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Citations

Mar 19, 2016·Journal of Molecular Histology·Min LianXingmei Feng
Apr 22, 2016·Journal of Molecular Histology·Hye-Kyung LeeJoo-Cheol Park
Apr 3, 2019·Journal of Molecular Histology·Jiameng WuXiaoying Wang

Related Concepts

FHL2 protein, human
Fhl2 protein, mouse
Teens
Metazoa
Bone Mineralization
Cell Differentiation Process
Dental Pulp
Molar Tooth
Muscle Proteins
Odontoblasts

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