FHOD formin and SRF promote post-embryonic striated muscle growth through separate pathways in C. elegans.

Experimental Cell Research
C. V. Yingling, David Pruyne

Abstract

Previous work with cultured cells has shown transcription of muscle genes by serum response factor (SRF) can be stimulated by actin polymerization driven by proteins of the formin family. However, it is not clear if endogenous formins similarly promote SRF-dependent transcription during muscle development in vivo. We tested whether formin activity promotes SRF-dependent transcription in striated muscle in the simple animal model, Caenorhabditis elegans. Our lab has shown FHOD-1 is the only formin that directly promotes sarcomere formation in the worm's striated muscle. We show here FHOD-1 and SRF homolog UNC-120 both support muscle growth and also muscle myosin II heavy chain A expression. However, while a hypomorphic unc-120 allele blunts expression of a set of striated muscle genes, these genes are largely upregulated or unchanged by absence of FHOD-1. Instead, pharmacological inhibition of the proteasome restores myosin protein levels in worms lacking FHOD-1, suggesting elevated proteolysis accounts for their myosin deficit. Interestingly, proteasome inhibition does not restore normal muscle growth to fhod-1(Δ) mutants, suggesting formin contributes to muscle growth by some alternative mechanism. Overall, we find SRF does no...Continue Reading

References

May 1, 1974·Genetics·S Brenner
Nov 6, 1998·Nature·L Timmons, A Fire
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·X ZhangJ Y Wei
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Francesca J DavisMahesh P Gupta
Jul 23, 2002·Nature Cell Biology·Isabelle SagotDavid Pellman
Oct 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Da-Zhi WangEric N Olson
Jan 15, 2003·Biochemistry·Martin PringSally H Zigmond
Oct 17, 2003·Current Biology : CB·Sally H ZigmondMartin Pring
Sep 17, 2004·The Journal of Biological Chemistry·John W CopelandRichard Treisman
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·David R Kovar, Thomas D Pollard
Dec 16, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Dean P StausChristopher P Mack
Mar 22, 2007·Annual Review of Biochemistry·Bruce L Goode, Michael J Eck
Jul 18, 2009·Journal of the American College of Cardiology·Luisa Mestroni
Aug 27, 2009·The Journal of Biological Chemistry·Kenichiro TaniguchiHideki Sumimoto
Apr 24, 2010·Nature Reviews. Molecular Cell Biology·Eric N Olson, Alfred Nordheim
May 26, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joseph M Miano
Nov 26, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Dean P StausChristopher P Mack
Aug 10, 2011·The Journal of Cell Biology·Pamela A Harvey, Leslie A Leinwand
Jan 19, 2012·PLoS Genetics·Timothy EtheridgeNathaniel J Szewczyk
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Dec 21, 2012·Circulation. Cardiovascular Genetics·Eric C WootenGordon S Huggins
Mar 5, 2013·Worm·Susann LehmannNathaniel J Szewczyk
Oct 4, 2013·Circulation Journal : Official Journal of the Japanese Circulation Society·Takuro ArimuraAkinori Kimura
Jan 17, 2014·Molecular Biology of the Cell·Michelle RosadoBruce L Goode
Feb 25, 2014·Redox Biology·Nikoletta Papaevgeniou, Niki Chondrogianni

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