Fiber composite slices for multiplexed immunoassays

Biomicrofluidics
Jiyun KimSunghoon Kwon

Abstract

Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads. The manufacturing process involves the fabrication of functionalized fibers and the arrangement of these fibers into a bundle; this bundle is then sectioned to make microarray-like particles with a predefined surface architecture. With these sections, composed of heterogeneous thread fragments with different types of antibodies, we demonstrated quantitative and 7-plex immunoassays. We expect that this methodology will prove to be a versatile, low-cost, and highly scalable method for the fabrication of multiplexed bioassay platforms.

References

Jul 16, 2002·Annual Review of Biomedical Engineering·Michael J Heller
Nov 28, 2002·Nature Genetics·Gavin MacBeath
Feb 6, 2003·Angewandte Chemie·David S Wilson, Steffen Nock
Dec 22, 2004·Mechanisms of Ageing and Development·Oliver PoetzThomas O Joos
Dec 24, 2004·Nature·Jingdong TianGeorge Church
Jan 27, 2005·Molecular & Cellular Proteomics : MCP·Brian B Haab
Jun 15, 2005·Annual Review of Biochemistry·Roland B Stoughton
Feb 18, 2006·Nature Reviews. Genetics·Jörg D Hoheisel
Aug 31, 2006·Angewandte Chemie·Robert WilsonDavid G Spiller
Mar 10, 2007·Science·Daniel C PregibonPatrick S Doyle
Jun 30, 2007·The International Journal of Biochemistry & Cell Biology·Eliezer Kopf, Dorit Zharhary
Mar 19, 2008·Nature Reviews. Genetics·David GreshamDavid Botstein
Sep 23, 2008·Analytical and Bioanalytical Chemistry·Michael HartmannThomas O Joos
May 19, 2009·Angewandte Chemie·Srijanani BhaskarJoerg Lahann
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sam Birtwell, Hywel Morgan
Jan 16, 2010·Clinical Chemistry·Xiaobo YuThomas O Joos
Apr 2, 2010·ACS Applied Materials & Interfaces·Xu LiWei Shen
May 26, 2010·ACS Applied Materials & Interfaces·Meital RechesGeorge M Whitesides
Aug 24, 2010·Nature Materials·Howon LeeSunghoon Kwon
Dec 4, 2010·Nature Protocols·Manlio Di CristinaAndrea Crisanti
Jan 19, 2011·Analytica Chimica Acta·Lin Wang, Paul C H Li
Jul 8, 2011·Lab on a Chip·Paridhi BhandariDhananjaya Dendukuri
Oct 22, 2011·Nature Protocols·David C AppleyardPatrick S Doyle
Nov 18, 2011·Lab on a Chip·Azadeh NilghazMohammed Rafiq Abdul Kadir
Aug 15, 2012·Analytical Chemistry·Gina ZhouDavid Juncker
Nov 12, 2013·Analytical Chemistry·Richard C MurdockJoshua A Hagen
Nov 19, 2013·Analytical Chemistry·Rathi L SrinivasPatrick S Doyle
Nov 14, 2014·Scientific Reports·Shang-Chi LinChao-Min Cheng

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Citations

Jan 15, 2019·ACS Combinatorial Science·Syamak FarajikhahGordon Wallace

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