Dec 24, 1997

Fiber-specific molecular features of tumors induced in rat peritoneum

Environmental Health Perspectives
K UnfriedW Dehnen

Abstract

Molecular markers such as mutational spectra or mRNA expression patterns may give some indication of the mechanisms of carcinogenesis induced by fibers and other carcinogens. In our study, tumors were induced by application of crocidolite asbestos or benzo[a]pyrene (B[a]P) to rat peritoneum. DNA and RNA of these tumors were subjected to analysis of point mutations and to investigation of mRNA expression patterns. With both assays we found typical features depending on the type of carcinogen applied. The analysis of point mutations in the tumor suppressor gene p53 revealed mutations in the B[a]P-induced tumors. However, in the tumors induced by crocidolite asbestos that were of the same tumor type as those induced by B[a]P, mutations in p53 were not detectable. Every mutation detected on the DNA level causes an amino acid substitution within one of the functional domains of the tumor suppressor protein. Therefore, these mutations seem to be of biological relevance for tumor progression and indicate a difference in the carcinogenesis regarding the type of the carcinogenic substance. An additional specificity of crocidolite-induced tumors was detectable by analyzing the mRNA expression of the tumor suppressor gene WT1, which is kn...Continue Reading

  • References35
  • Citations3

References

  • References35
  • Citations3

Citations

Mentioned in this Paper

TP53 gene
Tumor Promoters
Abdominal Neoplasms
SDS-PAGE
Mesothelioma
Neoplasms
Genetic Markers
Mineral Fibers
Benzo(a)pyrene
Asbestos, Crocidolite (Fe5Na2(SiO3)8)

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