PMID: 9172078Jun 1, 1997Paper

Fibre type-specific and nerve-dependent regulation of myosin light chain 1 slow promoter in regenerating muscle

Journal of Muscle Research and Cell Motility
R JerkovicS Schiaffino

Abstract

The regulation of a slow muscle gene, the myosin light chain 1 slow/ventricular gene, has been studied by in vivo transfection into regenerating rat skeletal muscle. Constructs containing portions of the myosin light chain 1 slow/ventricular promoter linked to reporter genes were injected into fast and slow muscles 3 days after muscle injury by bupivacaine injection, and reporter gene activity was analysed after 10 days. We report that a sequence in the 5' flanking region of the myosin light chain 1 slow/ventricular gene is able to direct slow muscle-specific regulation of reporter genes, and that the expression of the transgene, like that of the corresponding endogenous gene, is dependent on intact nerve. This study validates the use of regenerating muscle as a model for studying muscle gene regulation and is the first demonstration of a myosin gene promoter regulated by nerve activity.

Citations

Aug 26, 1998·Acta Physiologica Scandinavica·S SchiaffinoM Murgia
May 18, 1999·The American Journal of Physiology·M T WheelerS J Swoap
Jul 4, 2003·American Journal of Physiology. Cell Physiology·Anne BertrandDavid Tuil
Feb 6, 2010·Journal of Muscle Research and Cell Motility·Zaheer A RanaAndres Buonanno
Oct 21, 2011·Physiological Reviews·Stefano Schiaffino, Carlo Reggiani
Dec 12, 2002·The Journal of Biological Chemistry·Detlef Vullhorst, Andres Buonanno
Mar 9, 2000·Nature Cell Biology·M MurgiaS Schiaffino
Mar 7, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C H WashabaughM Ontell
Jun 6, 2000·American Journal of Physiology. Cell Physiology·P L HallauerK E Hastings
Mar 11, 1999·Current Opinion in Neurobiology·A Buonanno, R D Fields
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·J B Harris
Apr 29, 2021·American Journal of Respiratory Cell and Molecular Biology·Joseph BalnisAriel Jaitovich

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