Fibrillation of hen egg white lysozyme triggers reduction of copper(II)

International Journal of Biological Macromolecules
Sudeshna GhoshSwagata Dasgupta

Abstract

Copper is known to exert diverse effects on the self-association of proteins and has been found in amyloid deposits that are involved in neurodegenerative disease processes. The effects of the metal ion on the protein during fibrillation were investigated by fluorescence, circular dichroism spectroscopy and fluorescence microscopy. We report for the first time, the complete reduction of Cu(II)→Cu(I) in vitro during fibrillation of hen egg white lysozyme at pH 7. This was confirmed by the lack of any signal for Cu(II) in electron paramagnetic resonance spectroscopy and quantification of Cu(I) was achieved by a bathocuproine disulfonate based assay.

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Citations

Aug 21, 2013·International Journal of Biological Macromolecules·Nitin K PandeySwagata Dasgupta
Aug 13, 2013·Journal of Photochemistry and Photobiology. B, Biology·Sudeshna GhoshSwagata Dasgupta
Jun 4, 2014·Journal of Photochemistry and Photobiology. B, Biology·Sudeshna GhoshSwagata Dasgupta
Nov 6, 2012·International Journal of Biological Macromolecules·Weijuan HuangZhaocheng Ma
Dec 1, 2015·International Journal of Biological Macromolecules·Sumit K ChaturvediRizwan H Khan
Apr 19, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mingyang JingRutao Liu
Dec 12, 2012·International Journal of Biological Macromolecules·Sudeshna GhoshSwagata Dasgupta
Jul 23, 2013·Journal of Biomolecular Structure & Dynamics·Nitin Kumar PandeySwagata Dasgupta
Jan 20, 2021·Chemical Communications : Chem Comm·Antonello Merlino
Oct 19, 2013·The Journal of Physical Chemistry. B·Susmita BhattacharyaAnushree Roy
Nov 26, 2021·Journal of Biomolecular Structure & Dynamics·Ricardo CerónHugo Nájera

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