Fibrin-filled scaffolds for bone-tissue engineering: An in vivo study

Journal of Biomedical Materials Research. Part a
Jeffrey M KarpJohn E Davies

Abstract

Recently, fibrin sealants that typically contain supra physiological concentrations of fibrinogen and thrombin have been investigated as matrices to facilitate the delivery of cells within biodegradable scaffolds for tissue engineering applications. It is well known from in vitro experiments that the thrombin concentration present during fibrin polymerization influences the structural properties of fibrin, and these can affect cell invasion. This study was conducted to determine whether the structural properties of fibrin can affect bony wound healing in vivo. Drill hole defects were created in the distal femurs of 20 rats. Four experimental groups were used: nontreated defects, scaffolds alone, and scaffolds filled with fibrin polymerized with either a low thrombin concentration [fibrin(low T)] or a high thrombin concentration [fibrin(high T)]. The area of bone formed at 2, 5, and 11 days after implantation was determined histomorphometrically. After 5 days, scaffolds filled with fibrin(high T) were infiltrated with less bone than empty scaffolds (p < 0.05), but no statistical difference was found between the empty scaffolds and the scaffolds filled with fibrin(low T). After 11 days, both fibrin-filled scaffolds significantly ...Continue Reading

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Citations

Nov 24, 2006·Journal of Materials Science. Materials in Medicine·Eileen Gentleman, Julia M Polak
Jul 11, 2007·Journal of Materials Science. Materials in Medicine·Damien Le NihouannenGuy Daculsi
Dec 30, 2010·Tissue Engineering. Part a·Sang-Hyug ParkByoung-Hyun Min
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