Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium

Experimental Biology and Medicine
Jun LiHao Lai

Abstract

Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothelial cells and cardiomyocyte-like differentiation of BMSCs in vitro. Four weeks after transplantation of fibrin patch loaded with gene-modified BMSCs, IGF-1 overexpression could successfully promote angiogenesis, inhibit remodeling, increase grafted cell survival and reduce apoptosis. In conclusion, the integrated strategy, which combined fibrin patch with IGF-1 gene modified BMSCs, could promote the histological cardiac repair for a clinically relevant porcine model of I/R myocardium injury.

References

Sep 10, 1998·The Journal of Cell Biology·Z QuJ Huard
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Jingbo LiuJianyi Zhang
Feb 28, 2006·Tissue Engineering·Ge ZhangLaura Suggs
May 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael E DavisRichard T Lee
May 12, 2012·Stem Cell Reviews and Reports·Timothy J CashmanKevin D Costa
Sep 14, 2013·Circulation Research·Lei YeJianyi Zhang

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Citations

Oct 25, 2020·Stem Cell Reviews and Reports·Pallavi PushpMukesh Kumar Gupta

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Methods Mentioned

BETA
FACS
transfection

Software Mentioned

Syngo MMWP
Argus
SPSS

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