Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold

Journal of Biomaterials Science. Polymer Edition
Munirah Sha'banGilson Khang

Abstract

Previously, we have proven that fibrin and poly(lactic-co-glycolic acid) (PLGA) scaffolds facilitate cell proliferation, matrix production and early chondrogenesis of rabbit articular chondrocytes in in vitro and in vivo experiments. In this study, we evaluated the potential of fibrin/PLGA scaffold for intervertebral disc (IVD) tissue engineering using annulus fibrosus (AF) and nucleus pulposus (NP) cells in relation to potential clinical application. PLGA scaffolds were soaked in cells-fibrin suspension and polymerized by dropping thrombin-sodium chloride (CaCl(2)) solution. A PLGA-cell complex without fibrin was used as control. Higher cellular proliferation activity was observed in fibrin/PLGA-seeded AF and NP cells at each time point of 3, 7, 14 and 7 days using the MTT assay. After 3 weeks in vitro incubation, fibrin/PLGA exhibited a firmer gross morphology than PLGA groups. A significant cartilaginous tissue formation was observed in fibrin/PLGA, as proven by the development of cells cluster of various sizes and three-dimensional (3D) cartilaginous histoarchitecture and the presence of proteoglycan-rich matrix and glycosaminoglycan (GAG). The sGAG production measured by 1,9-dimethylmethylene blue (DMMB) assay revealed gre...Continue Reading

References

Feb 24, 2001·Acta Orthopaedica Scandinavica·S SternC Perka
Aug 9, 2003·Advances in Experimental Medicine and Biology·Gilson KhangHai Bang Lee
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·Satoru YamazakiJoe T Minchew
Dec 18, 2004·Biomaterials·Grace E ParkThomas J Webster
Sep 12, 2006·Biomaterials·Daniela EyrichTorsten Blunk
Sep 15, 2006·Journal of Zhejiang University. Science. B·Yi-xiong ZhengMichael Sittinger
Feb 13, 2007·Cell Biology International·M FröhlichN Kregar Velikonja
May 9, 2008·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Suresh K BhagatMary S Mathews
Jul 22, 2008·Artificial Organs·Antoine P SimonsJos G Maessen

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Citations

Jul 9, 2011·Tissue Engineering. Part a·Sang-Hyug ParkDavid L Kaplan
Sep 17, 2011·Tissue Engineering. Part a·Sang-Hyug ParkDavid L Kaplan
Jun 18, 2014·Regenerative Medicine·Zhen LiAvner Yayon
Jun 26, 2014·Tissue Engineering. Part B, Reviews·Alessandra ColombiniMatteo Moretti
Aug 17, 2011·The Spine Journal : Official Journal of the North American Spine Society·Minting YuanBarbara P Chan
Aug 8, 2015·International Journal of Pediatric Otorhinolaryngology·Mohamad Fikeri Bin IshakRuszymah Bt Haji Idrus
Mar 13, 2014·Journal of Tissue Engineering and Regenerative Medicine·Shahriar SharifiRoel Kuijer
Jul 8, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Márcia A RebeloMarco V Chaud
Jul 5, 2016·Journal of Tissue Engineering and Regenerative Medicine·Sébastien B G BlanquerSuvi P Haimi
Feb 20, 2018·Tissue Engineering. Part C, Methods·Anita KrouwelsLaura B Creemers
Jun 7, 2017·Bone Research·Mei LiuNongyue He
Jan 17, 2012·Journal of Biomaterials Science. Polymer Edition·Chengzhen LiangQixin Chen
Jun 3, 2020·Advanced Healthcare Materials·Maria Grazia RaucciLuigi Ambrosio
Mar 8, 2017·Tissue Engineering and Regenerative Medicine·Javad Tavakoli
Mar 13, 2014·Journal of Tissue Engineering and Regenerative Medicine·Hye Yun KimGilson Khang

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