Fibrinogen adsorption on three silica-based surfaces: conformation and kinetics

Langmuir : the ACS Journal of Surfaces and Colloids
Alicia Toscano, Maria M Santore

Abstract

Using AFM (atomic force microscopy) to probe protein conformation and arrangement, and TIRF (total internal reflectance fluorescence) to monitor kinetics, fibrinogen adsorption on three different silica-based surfaces was studied: the native oxide on silicon, acid-etched microscope slides, and acid-etched polished glass. The three are chemically similar, but the microscope slide is rougher and induces AFM tip instabilities that appear as high spots on the bare surface. Fibrinogen's conformation and transport-limited adsorption kinetics are found to be quantitatively similar on all three surfaces. Further, the number of adsorbed proteins in progressive AFM micrographs quantitatively match the coverages measured by TIRF during early adsorption. Surfaces appear full, via AFM, when adsorbed amounts are about an order of magnitude below their true saturation levels (via TIRF) because, above about 0.26 mg/m(2), individual proteins cannot be discerned. The results demonstrate how the appearance of AFM micrographs can be misleading regarding surface saturation. On all three surfaces, fibrinogen is, at most, slightly aggregated, showing limited, if any, surface mobility. The complexities of the microscope slide's surface landscape minim...Continue Reading

References

Mar 25, 1991·FEBS Letters·R WigrenI Lundström
Jul 6, 2000·Journal of Biomedical Materials Research·N B Holland, R E Marchant
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·C E HALL, H S SLAYTER
Sep 24, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Aashiish Agnihotri, Christopher A Siedlecki
Feb 8, 2005·Ultramicroscopy·Aashiish Agnihotri, Christopher A Siedlecki

❮ Previous
Next ❯

Citations

May 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zbigniew AdamczykMichał Cieśla
Oct 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zbigniew AdamczykMałgorzata Nattich-Rak
Nov 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·K H Aaron LauPhillip B Messersmith
May 26, 2010·The Analyst·Laurel E Averett, Mark H Schoenfisch
Feb 5, 2014·Journal of Colloid and Interface Science·Arianna MaruccoIvana Fenoglio
Apr 23, 2010·Biointerphases·Bogdan ZdyrkoIgor Luzinov
Mar 22, 2014·Soft Matter·David A Woods, Colin D Bain
Oct 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Maria Dąbkowska, Zbigniew Adamczyk
Feb 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Anna Bratek-SkickiMichał Cieśla
Apr 30, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Michał CieślaMonika Wasilewska
Aug 14, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Małgorzata Nattich-RakMarta Sadowska
Dec 27, 2015·Colloids and Surfaces. B, Biointerfaces·Katarzyna KubiakMichał Cieśla
Dec 25, 2012·Colloids and Surfaces. B, Biointerfaces·Anna Bratek-SkickiŻbigniew Adamczyk
Aug 14, 2012·Journal of Colloid and Interface Science·Zbigniew AdamczykMonika Wasilewska
May 31, 2011·Advances in Colloid and Interface Science·Z AdamczykM Zaucha
Feb 15, 2011·Journal of Colloid and Interface Science·Z AdamczykM Sadowska
Sep 14, 2010·Colloids and Surfaces. B, Biointerfaces·M Reza Nejadnik, Carlos D Garcia
Jun 27, 2009·Colloids and Surfaces. B, Biointerfaces·Surachate Kalasin, Maria M Santore
Nov 17, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Stephan KöhlerGiovanni Settanni
Feb 26, 2008·Micron : the International Research and Review Journal for Microscopy·Pranav SomanChristopher A Siedlecki
May 8, 2009·Macromolecular Bioscience·José Carlos Rodríguez HernándezManuel Salmerón-Sánchez
Jan 18, 2007·Journal of Biomedical Materials Research. Part a·Cristèle J NonckremanChristine C Dupont-Gillain
Nov 30, 2006·Journal of Biomedical Materials Research. Part a·Robert T T Gettens, Jeremy L Gilbert
Apr 22, 2011·Macromolecular Rapid Communications·Bogdan Zdyrko, Igor Luzinov
Apr 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Yu HuWei Jiang
Jul 26, 2015·Journal of Colloid and Interface Science·Katarzyna KubiakMonika Wasilewska
Dec 3, 2014·Journal of Colloid and Interface Science·Małgorzata Nattich-RakMarta Sadowska
Feb 8, 2011·Advances in Colloid and Interface Science·Michael RabeStefan Seeger
Oct 27, 2016·Soft Matter·Anna Bratek-SkickiJuan M Ruso
Jan 6, 2017·Annals of Biomedical Engineering·Yusuke InoueMasaki Sekino
Jun 3, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Ramya KumarJoerg Lahann
Mar 5, 2014·Journal of Dental Research·D VukosavljevicW L Siqueira
Jun 24, 2009·Nanotechnology·Ling YuChang Ming Li
Jun 4, 2008·Physical Review Letters·Katja HenzlerMatthias Ballauff
Sep 4, 2008·Biomedical Materials·M YaseenJ R Lu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Langmuir : the ACS Journal of Surfaces and Colloids
Aashiish Agnihotri, Christopher A Siedlecki
Journal of the American Chemical Society
Seung-Yong JungPaul S Cremer
Langmuir : the ACS Journal of Surfaces and Colloids
Maria M Santore, Christian F Wertz
Langmuir : the ACS Journal of Surfaces and Colloids
Zbigniew AdamczykMichał Cieśla
© 2021 Meta ULC. All rights reserved