Fibrinogen adsorption to receptor-like biomaterials made by pre-adsorbing peptides to polystyrene substrates

Journal of Molecular Recognition : JMR
John M Grunkemeier, Thomas A Horbett

Abstract

Two peptides from the ligand-binding site of the platelet receptor GPIIb/IIIa, residues 296-306 of GPIIb, designated B12 by D'Souza et al. (1991), and 300-311 of GPIIb, designated G13 by Taylor et al., (1992), as well as two control peptides, designated C14 and C20, were adsorbed to treated polystyrene substrates. Fibrinogen adsorption to the peptide-coated substrates was characterized. The specificity of I-125 labeled fibrinogen binding to the peptide-coated substrates was investigated by measuring the amount of fibrinogen adsorbed to each substrate and the inhibition of fibrinogen binding by RGDS peptide, bovine serum albumin, a divalent ion chelator (ethylene diamine tetra-acetic acid disodium salt), unlabeled fibrinogen and the B12 peptide. The results show that non-specific binding of fibrinogen to the B12-coated substrate is predominant under most conditions. Binding of monoclonal antibodies to fibrinogen adsorbed to the peptide coated substrates was characterized. The failure of several antibodies to bind fibrinogen adsorbed to the B12 substrate suggested that adsorption of fibrinogen to the B12-coated substrate alters its conformation relative to fibrinogen adsorbed to the bare substrate.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D H FarrellE W Davie
Dec 1, 1991·Journal of Biomedical Materials Research·W R GombotzA S Hoffman
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·P S CraigT Romig
Jun 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M B Zucker, L Vroman
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·T A Horbett, K R Lew
Mar 1, 1993·Journal of Biomedical Materials Research·B D Ratner
Jan 1, 1993·Journal of Biomaterials Science. Polymer Edition·G R Llanos, M V Sefton
Apr 1, 1993·Analytical Biochemistry·T J Tsomides, H N Eisen

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Citations

Jun 29, 2007·Journal of Materials Science. Materials in Medicine·A K Bajpai
Apr 20, 2007·Journal of Materials Science. Materials in Medicine·Paul RoachCarole C Perry
May 4, 2001·Journal of Biomaterials Science. Polymer Edition·J M GrunkemeierT A Horbett
Dec 5, 2002·Journal of Biomaterials Science. Polymer Edition·Rumiana TzonevaDieter Paul
Oct 16, 2008·Journal of Biomaterials Science. Polymer Edition·Min ZhangThomas A Horbett
May 23, 2008·Journal of Biomedical Materials Research. Part a·Min Zhang, Thomas A Horbett

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