PMID: 6017979Feb 24, 1967Paper

Fibrinogen from human plasma: preparation by precipitation with heavy-metal coordination complex

Science
M E Brown, F Rothstein

Abstract

Potassium tetrathiocyanato-(S)mercurate II [K(2)Hg(SCN)(4)] is used in a mild and rapid procedure for the isolation of human fibrinogen from fresh plasma. The final product, 94 to 99 percent of which is coagulable by thrombin, represents an average yield of 80 percent and is stable in solution. It is free of plasmin, streptokinase-activatable plasminogen, and coagulation factors II, V, VIII, X and XIII. Sedimentation analysis reveals a single peak with a sedimentation coefficient equal to 7.0S at infinite dilution. Immunodiffusion on cellulose acetate results in two precipitin lines with rabbit antiserum to whole human serum. The fibrinogen precipitates are unusual in that they are flocculent and readily redissolve.

References

Apr 1, 1966·American Journal of Clinical Pathology·C A Saravis
Dec 28, 1966·Journal of Molecular Biology·C CohenC Hall
Nov 1, 1962·The Journal of Experimental Medicine·J R SHAINOFF, I H PAGE
Feb 26, 1962·Biochimica Et Biophysica Acta·M W MOSESSON
Jul 1, 1951·Archives of Biochemistry and Biophysics·K LAKI

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Citations

Apr 2, 1974·Archives of Biochemistry and Biophysics·P C Leavis, F Rothstein
Feb 25, 1977·Journal of Molecular Biology·N M Tooney, C Cohen
Oct 23, 1970·Science·S E Charm, B L Wong

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