Fibrinogen Release and Deposition on Urinary Catheters Placed during Urological Procedures

The Journal of Urology
Ana L Flores-MirelesAlana Desai

Abstract

Catheter associated urinary tract infections account for approximately 40% of all hospital acquired infections worldwide with more than 1 million cases diagnosed annually. Recent data from a catheter associated urinary tract infection animal model has shown that inflammation induced by catheterization releases host fibrinogen, which accumulates on the catheter. Further, Enterococcus faecalis catheter colonization was found to depend on EbpA (endocarditis and biofilm-associated pilus), a fibrinogen binding adhesin. We evaluated this mechanism in a human model. Urinary catheters were collected from patients hospitalized for surgical or nonsurgical urological procedures. Catheters were subjected to immunofluorescence analyses by incubation with antifibrinogen antibody and then staining for fluorescence. Fluorescence intensity was compared to that of standard catheters. Catheters were incubated with strains of Enterococcus faecalis, Staphylococcus aureus or Candida to assess binding of those strains to fibrinogen laden catheters. After various surgical and urological procedures, 50 catheters were collected. In vivo dwell time ranged from 1 hour to 59 days. All catheters had fibrinogen deposition. Accumulation depended on dwell time...Continue Reading

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Citations

Mar 22, 2016·Pathogens·Caitlin N Spaulding, Scott J Hultgren
Jan 20, 2017·Virulence·H M Sharon GohKimberly A Kline
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jennifer N WalkerScott J Hultgren
Nov 1, 2016·MBio·Ana L Flores-MirelesMichael G Caparon
Jul 2, 2019·Aesthetic Surgery Journal·Jennifer N WalkerTerence M Myckatyn
Sep 25, 2019·Topics in Spinal Cord Injury Rehabilitation·Ana Flores-MirelesDavid A Hunstad
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Mar 19, 2019·Plastic and Reconstructive Surgery. Global Open·Jennifer N WalkerTerence M Myckatyn
Feb 20, 2020·Nature Reviews. Microbiology·Roger D Klein, Scott J Hultgren
May 21, 2019·Bio-protocol·Cristina Colomer-WinterAna L Flores-Mireles
Nov 6, 2020·Cells·Ermioni Kalfopoulou, Johannes Huebner
Jun 1, 2021·ACS Omega·Buddhika GayaniManjula Manoji Weerasekera

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