Fibroblast dependency during early thymocyte development maps to the CD25+ CD44+ stage and involves interactions with fibroblast matrix molecules

European Journal of Immunology
Graham AndersonEric J Jenkinson

Abstract

We have investigated the role of specific components of the thymic stroma during development of CD4-8-T cell precursors by separating and reaggregating precursor subsets with individual or combinations of stromal cells. We show that while the development of CD25+ 44+ precursors is dependent upon a combination of major histocompatibility complex (MHC) class II+ thymic epithelial cells and fibroblasts, their direct descendants, CD25+ 44- precursors, develop to the CD4+ 8+ stage in the presence of MHC class II+ thymic epithelial cells alone. Thus, CD25+ 44+ precursors are the last developmental stage to be dependent upon fibroblast support. In addition, while metabolically inactive, 1-ethyl-3-(3'-dimethylaminopropyl) carbodiimide (ECDI)-treated fibroblasts retain the ability to promote T cell development, prior treatment with hyaluronidase abrogates this effect, suggesting that fibroblast-associated extracellular matrix components are the key elements involved. In support of this, we show that fibroblasts are located in cortical regions of the thymus where T cell precursors are known to reside, and that these fibroblasts are associated with an extensive extracellular matrix not found on thymic epithelial cells. Finally, antibodies...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K UtsumiM Ogata
Jan 1, 1991·Annual Review of Immunology·W van Ewijk
Nov 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Lannes-VieiraW Savino
Mar 1, 1991·The Journal of Experimental Medicine·K MiyakeP W Kincade
Feb 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Jan 1, 1987·Advances in Immunology·P W Kincade
Jul 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S BerrihS Cohen
Jul 1, 1985·European Journal of Immunology·E Van VlietW Van Ewijk
Dec 1, 1993·Immunology Today·M Groettrup, H von Boehmer
Jun 1, 1994·The Journal of Experimental Medicine·G AndersonE J Jenkinson
Jan 1, 1993·Advances in Immunology·J LesleyP W Kincade
Apr 1, 1993·Current Opinion in Immunology·A M Kruisbeek
Mar 1, 1993·Immunology Today·C R Mackay, B A Imhof
Jan 1, 1996·Annual Review of Immunology·G AndersonE J Jenkinson

❮ Previous
Next ❯

Citations

Oct 10, 2001·European Journal of Immunology·C SchwärzlerU Günthert
Apr 5, 2002·European Journal of Immunology·Claudia Hetzer-EggerThomas Boehm
Jan 17, 2002·Journal of Immunological Methods·D H D GrayR L Boyd
Aug 6, 1998·Journal of Immunological Methods·M G KimR H Schwartz
Oct 6, 1998·Journal of Immunological Methods·G Anderson, E J Jenkinson
Aug 1, 1997·Immunology Today·G Anderson, E Jenkinson
Jun 20, 2002·Trends in Immunology·Wilson SavinoVinícius Cotta-de-Almeida
Mar 22, 2002·Nature Reviews. Immunology·G Anderson, E J Jenkinson
Mar 23, 2000·The Journal of Experimental Medicine·R K SuniaraJ J Owen
Jul 16, 2003·The Journal of Experimental Medicine·William E JenkinsonGraham Anderson
Jul 13, 2007·International Immunology·Manami ItoiTakashi Amagai
Mar 24, 2005·Neuroimmunomodulation·Wilson SavinoMireille Dardenne
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D B KlugE R Richie
Mar 7, 2002·Journal of Neuroimmunology·Ji Ming FengAnthony T Campagnoni
Dec 1, 2001·Expert Opinion on Biological Therapy·B Bodey
Sep 14, 1999·Pathology, Research and Practice·M InoueH Matsuda
Feb 25, 2009·Annals of the New York Academy of Sciences·Daniella Arêas Mendes-da-CruzWilson Savino
May 23, 2014·Biochemical Society Transactions·Richard AspinallPierre O Lang
Apr 19, 2016·Immunological Reviews·Beth LucasGraham Anderson
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J J OwenE J Jenkinson
Aug 10, 2006·European Journal of Immunology·Judit E PongraczEric J Jenkinson
Feb 2, 2006·Immunological Reviews·Graham AndersonEric J Jenkinson
Oct 18, 2002·Blood·Matthew EricksonAndrew G Farr
Mar 3, 2007·European Journal of Immunology·Victoria A GrahamDavid F Tough
Feb 5, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chacko JosephLouise E Purton
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel H D GrayRichard L Boyd
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susan E ProckopHoward T Petrie
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H T PetrieF Livak
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M NormentM J Bevan
Sep 13, 2003·Immunological Reviews·Jason GillRichard Boyd
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D M Su, N R Manley
Sep 13, 2003·Immunological Reviews·Thomas BoehmMichael Schorpp
Feb 2, 2021·Frontiers in Immunology·Takeshi Nitta, Hiroshi Takayanagi
Feb 19, 2021·The Journal of Biological Chemistry·Hsuan-Po HsuIvan L Dzhagalov
Mar 16, 2021·Frontiers in Immunology·Kieran D JamesGraham Anderson
Jun 8, 2021·Immunological Reviews·Takeshi NittaHiroshi Takayanagi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.