Fibroblast growth factor-1 induced promatrilysin expression through the activation of extracellular-regulated kinases and STAT3

Neoplasia : an International Journal for Oncology Research
T S UdayakumarGeorge Timothy Bowden

Abstract

The MMP, matrilysin (MMP-7), has been shown to be overexpressed in prostate cancer cells and to increase prostate cancer cell invasion. Prostate stromal fibroblasts secrete factor(s), including fibroblast growth factor-1 (FGF-1) that induces promatrilysin expression in LNCaP cells. In the present study, we investigated the signal transduction pathway involved in the FGF-1-induced expression of promatrilysin. FGF-1 treatment significantly increased the activation of extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2). This induction was time-dependent and was sustained until 24 hours after treatment. Treating the cells with MEK1/2 inhibitor (PD98059) eliminated ERK activation completely and blocked FGF-1-mediated induction of promatrilysin expression. Transient transfection studies with human matrilysin promoter resulted in a four- to five-fold increase in reporter luciferase enzyme activity that was blocked by the MEK1/2 inhibitor (PD98059). Serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was observed after FGF-1 treatment and pretreatment with 20 microM PD98059-abolished STAT3 phosphorylation. Transient transfection with dominant negative STAT3 inhibited FGF-1-induced transactiva...Continue Reading

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Citations

Oct 21, 2004·Cancer·David G BostwickBarry Timms
Oct 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Man-Man KangZheng-Jun Wang
May 12, 2005·Médecine sciences : M/S·Lionel Rémy, Cécile Trespeuch
Apr 7, 2006·American Journal of Physiology. Renal Physiology·Dianzhong ZhangJames A Bassuk
May 16, 2020·Journal of Oncology·Emily L PatonIsabel R Schlaepfer
Jul 31, 2020·Physiological Reviews·Arianna GiacominiMarco Presta
Jun 3, 2017·Experimental Cell Research·Nicoletta GaglianoPatrizia Procacci
Mar 5, 2004·Chemistry and Physics of Lipids·Yong CuiKyoung-Chan Park
Oct 16, 2004·Immunobiology·Bernard Burke
Feb 24, 2006·Biochemical and Biophysical Research Communications·Wolfgang HolnthonerPeter Petzelbauer

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